Anti-nNOS (neuronal) antibody (ab6175)

Overview

• Product nameAnti-nNOS (neuronal) antibodySee all nNOS (neuronal) primary antibodies ...
• Description
  Sheep polyclonal to nNOS (neuronal)
• SpecificityReacts with nNOS. Cross-reactivity with iNOS or eNOS not known, but not expected.
• Tested applicationsWB, IHC-P, IHC-Fr more details
• Species reactivity
  Reacts with: Rat, Rabbit, Guinea pig
  
• Immunogen

  Synthetic peptide: RSESIAFIEESKKDADEVFSS conjugated to KLH by a Glutaraldehyde linker, corresponding to amino acids 1409-1429 of Rat NOS1 (nNOS).

  Run BLAST with Run BLAST with
• Positive controlrat brain
• General notesThis antibody is a high quality immunohistochemical reagent that has been used for studies in both the peripheral and central nervous system. It is also useful for double label studies when combined with rabbit or other primary antibodies.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C (add glycerol to a final volume of 50% for extra stability). Avoid repeated freeze / thaw cycles.
• PurityWhole antiserum
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Hypoxia

  • Cancer
  • Cancer Metabolism
  • Response to hypoxia

  • Neuroscience
  • Neurotransmission
  • Nitric Oxide
  • NOS

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Parkinson's disease
  • Other

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Other

Applications

Target

• FunctionProduces nitric oxide (NO) which is a messenger molecule with diverse functions throughout the body. In the brain and peripheral nervous system, NO displays many properties of a neurotransmitter. Probably has nitrosylase activity and mediates cysteine S-nitrosylation of cytoplasmic target proteins such SRR.
• Tissue specificityIsoform 1 is ubiquitously expressed: detected in skeletal muscle and brain, also in testis, lung and kidney, and at low levels in heart, adrenal gland and retina. Not detected in the platelets. Isoform 3 is expressed only in testis. Isoform 4 is detected in testis, skeletal muscle, lung, and kidney, at low levels in the brain, but not in the heart and adrenal gland.
• Sequence similaritiesBelongs to the NOS family.
  Contains 1 FAD-binding FR-type domain.
  Contains 1 flavodoxin-like domain.
  Contains 1 PDZ (DHR) domain.
• DomainThe PDZ domain in the N-terminal part of the neuronal isoform participates in protein-protein interaction, and is responsible for targeting nNos to synaptic membranes in muscles. Mediates interaction with VAC14.
• Post-translational
  modificationsUbiquitinated; mediated by STUB1/CHIP in the presence of Hsp70 and Hsp40 (in vitro).
• Cellular localizationCell membrane > sarcolemma. Cell projection > dendritic spine. In skeletal muscle, it is localized beneath the sarcolemma of fast-twitch muscle fiber by associating with the dystrophin glycoprotein complex. In neurons, enriched in dendritic spines.

Target information above from: UniProt accession P29475 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 100009243 Rabbit
  • Entrez Gene: 24598 Rat
  • SwissProt: O19132 Rabbit
  • SwissProt: P29476 Rat
  • Unigene: 10573 Rat

• Alternative names
  2310005C01Rik antibodybNOS antibodyConstitutive NOS antibody

  EC 1.14.13.39 antibodyIHPS 1 antibodyIHPS1 antibodyN NOS antibodyN-NOS antibodyNC NOS antibodyNC-NOS antibodyNCNOS antibodyneuronal Nitric Oxide Synthase antibodyNeuronal NOS antibodyNitric oxide synthase , neuronal, included antibodyNitric oxide synthase 1 (neuronal) antibodyNitric oxide synthase 1 antibodyNitric oxide synthase brain antibodyNitric oxide synthase, brain antibodyNitric oxide synthase, penile neuronal, included antibodynNOS antibodyNO antibodyNOS 1 antibodyNOS antibodyNOS type I antibodyNOS-I antibodyNOS1 antibodyNOS1_HUMAN antibodyPeptidyl-cysteine S-nitrosylase NOS1 antibody

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Anti-nNOS (neuronal) antibody images

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  Immunohistochemistry (Frozen sections) - nNOS (neuronal) antibody (ab6175)
  Rat braistem staining for nNOS with ab6175 (1:2000) (perfusion fixed frozen sections).