Overview
- Product nameAnti-Prostaglandin E Synthase antibodySee all Prostaglandin E Synthase primary antibodies ...
- DescriptionRabbit polyclonal to Prostaglandin E Synthase
- Tested applicationsWB, IHC-P more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rabbit, Hamster, Cow, Pig, Xenopus laevis, Monkey, Gorilla, Marmoset (common)
- Immunogen
Synthetic peptide conjugated to KLH, corresponding to 13 amino acids from the 1st cytoplasmic domain of Human Prostaglandin E Synthase.
- Positive controlHuman testis tissue.
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.1% Sodium Azide
Constituents: PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab62049 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use at an assay dependent dilution. |
| IHC-P | IHC-P: Use a concentration of 10 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
- FunctionCatalyzes the oxidoreduction of prostaglandin endoperoxide H2 (PGH2) to prostaglandin E2 (PGE2).
- Sequence similaritiesBelongs to the MAPEG family.
- Cellular localizationMembrane.
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Database links
- Entrez Gene: 282019 Cow
- Entrez Gene: 101144874 Gorilla
- Entrez Gene: 9536 Human
- Entrez Gene: 100384917 Marmoset (common)
- Entrez Gene: 64292 Mouse
- Omim: 605172 Human
- SwissProt: O14684 Human
- SwissProt: Q9JM51 Mouse
- Unigene: 146688 Human
- Unigene: 28768 Mouse
see all
Target information above from: UniProt accession
O14684
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- Glutathione S transferase 1 like 1 antibodyMGC10317 antibodyMGST IV antibody
- MGST1 L1 antibodyMGST1 like 1 antibodyMGST1-L1 antibodyMGST1L1 antibodyMGSTIV antibodyMicrosomal glutathione S transferase 1 like 1 antibodyMicrosomal glutathione S-transferase 1-like 1 antibodyMicrosomal prostaglandin E synthase 1 antibodyMicrosomal prostaglandin E synthase 1 antibodymPGES 1 antibodyMPGES antibodymPGES-1 antibodyp53 induced apoptosis protein 12 antibodyp53 induced gene 12 antibodyp53 induced gene 12 protein antibodyp53-induced gene 12 protein antibodyPGES antibodyPIG 12 antibodyPIG12 antibodyPP 102 antibodyPP 1294 antibodyPP102 antibodyPP1294 antibodyProstaglandin E synthase antibodyPTGES antibodyPTGES_HUMAN antibodyTP53I12 antibodyTumor protein p53 inducible protein 12 antibody
see all
Anti-Prostaglandin E Synthase antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Prostaglandin E Synthase antibody (ab62049)Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue, staining Prostaglandin E Synthase with ab62049 at 10 µg/ml. -
Western blot - Prostaglandin E Synthase antibody (ab62049)Anti-Prostaglandin E Synthase antibody (ab62049) at 1/500 dilution (Incubation for 1 hour at 22°C in 5% Marvel TBS-T) + Whole cell lysate of mouse NIH-3T3 cells at 20 µg
Secondary
An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 19 kDa (why is the actual band size different from the predicted?)
Exposure time : 8 minutesThis image is courtesy of an anonymous Abreview
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Western blot - Prostaglandin E Synthase antibody (ab62049)Anti-Prostaglandin E Synthase antibody (ab62049) at 1/500 dilution (in 5% Marvel TBS-T for 1 hour at 22°C) + Whole cell lysate of human A549 cells at 20 µg
Secondary
An HRP-conjugated Goat anti-rabbit IgG polyclonal at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Observed band size : 19 kDa (why is the actual band size different from the predicted?)
Exposure time : 8 minutesThis image is courtesy of an anonymous Abreview
References for Anti-Prostaglandin E Synthase antibody (ab62049)
ab62049 has not yet been referenced specifically in any publications.
