Overview
- Product nameAnti-GFAP (phospho S38) antibodySee all GFAP primary antibodies ...
- DescriptionRabbit polyclonal to GFAP (phospho S38)
- Specificityab62479 detects endogenous levels of GFAP only when phosphorylated at serine 38.
- Tested applicationsELISA, IHC-P, IHC-Fr more details
- Species reactivityReacts with: Mouse, Human
- Immunogen
Synthetic phosphopeptide derived from human GFAP around the phosphorylation site of serine 38 (R-L-SP-L-A).
- Positive controlHuman brain tissue.
Properties
- FormLiquid
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS (without Mg2+ and Ca2+), 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesab62479 was affinity purified from rabbit antiserum by affinity chromatography using epitope specific phosphopeptide. The antibody against non phosphopeptide was removed by chromatography using non phosphopeptide corresponding to the phosphorylation site.
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab62479 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA: 1/5000. |
| IHC-P | IHC-P: 1/50 - 1/100. |
| IHC-Fr | IHC-Fr: 1/100 - 1/200. |
Target
- FunctionGFAP, a class-III intermediate filament, is a cell-specific marker that, during the development of the central nervous system, distinguishes astrocytes from other glial cells.
- Tissue specificityExpressed in cells lacking fibronectin.
- Involvement in diseaseDefects in GFAP are a cause of Alexander disease (ALEXD) [MIM:203450]. Alexander disease is a rare disorder of the central nervous system. It is a progressive leukoencephalopathy whose hallmark is the widespread accumulation of Rosenthal fibers which are cytoplasmic inclusions in astrocytes. The most common form affects infants and young children, and is characterized by progressive failure of central myelination, usually leading to death usually within the first decade. Infants with Alexander disease develop a leukoencephalopathy with macrocephaly, seizures, and psychomotor retardation. Patients with juvenile or adult forms typically experience ataxia, bulbar signs and spasticity, and a more slowly progressive course.
- Sequence similaritiesBelongs to the intermediate filament family.
- Post-translational
modificationsPhosphorylated by PKN1. - Cellular localizationCytoplasm. Associated with intermediate filaments.
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Database links
- Entrez Gene: 2670 Human
- Entrez Gene: 14580 Mouse
- Omim: 137780 Human
- SwissProt: P14136 Human
- SwissProt: P03995 Mouse
- Unigene: 514227 Human
- Unigene: 1239 Mouse
Target information above from: UniProt accession
P14136
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Astrocyte antibodyFLJ42474 antibodyFLJ45472 antibody
- GFAP antibodyGFAP_HUMAN antibodyGlial Fibrillary Acidic Protein antibodyIntermediate filament protein antibody
see all
Anti-GFAP (phospho S38) antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - GFAP (phospho S38) antibody (ab62479)ab62479, at 1/50 dilution, staining GFAP in paraffin embedded human brain tissue by Immunohistochemsitry in the absence (left image) or presence (right image) of the immunising peptide. -
Immunohistochemistry (Frozen sections) - GFAP (phospho S38) antibody (ab62479)This image is a courtesy of Anonymous Abreviewab62479 staining GFAP (phospho S38) in mouse brain tissue section by Immunohistochemistry (Frozen sections). Tissue samples were fixed with paraformaldehyde and blocking with a dilution buffer containing 2% Block Ace, 0.05% Triton X-100 and PBS was performed at 250C for 1 hour. The sample was incubated with primary antibody (1/100) at 40C for 12 hours. A Cyt3 ® -conjugated donkey polyclonal to rabbit IgG was used as secondary antibody at 1/500 dilution.
References for Anti-GFAP (phospho S38) antibody (ab62479)
ab62479 has not yet been referenced specifically in any publications.
