Overview
- Product nameAnti-ASK1 antibodySee all ASK1 primary antibodies ...
- DescriptionRabbit polyclonal to ASK1
- Tested applicationsWB, ICC/IF, ICC more details
- Species reactivityReacts with: Mouse, Human
- Immunogen
A 16 amino acid peptide from near the carboxy terminus of human ASK1
- Positive controlSW1353 cell lysate and A431 cells.
Properties
- FormLiquid
- Storage instructionsStore at +4°C.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: PBS -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab62575 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 155 kDa (predicted molecular weight: 155 kDa). |
| ICC/IF | ICC/IF: Use a concentration of 20 µg/ml. |
| ICC | ICC: Use a concentration of 2 µg/ml. |
Target
- FunctionComponent of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively. Overexpression induces apoptotic cell death.
- Tissue specificityAbundantly expressed in heart and pancreas.
- Sequence similaritiesBelongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 protein kinase domain. - Post-translational
modificationsDephosphorylated and activated by PGAM5. -
Database links
- Entrez Gene: 4217 Human
- Entrez Gene: 26408 Mouse
- Omim: 602448 Human
- SwissProt: Q99683 Human
- SwissProt: O35099 Mouse
- Unigene: 186486 Human
- Unigene: 6595 Mouse
Target information above from: UniProt accession
Q99683
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- Apoptosis signal regulating kinase 1 antibodyApoptosis signal-regulating kinase 1 antibodyASK 1 antibody
- ASK-1 antibodyASK1 antibodyM3K5 antibodyM3K5_HUMAN antibodyMAP/ERK kinase kinase 5 antibodyMAP3K5 antibodyMAPK/ERK kinase kinase 5 antibodyMAPKKK5 antibodyMEK kinase 5 antibodyMEKK 5 antibodyMEKK5 antibodyMitogen activated protein kinase kinase kinase 5 antibodyMitogen-activated protein kinase kinase kinase 5 antibody
see all
Anti-ASK1 antibody images
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Immunocytochemistry - ASK1 antibody (ab62575)ab62575 at 2µg/ml staining ASK1 in A431 cells by Immunocytochemistry. -
Western blot - ASK1 antibody (ab62575)Lane 1 : Anti-ASK1 antibody (ab62575) at 0.5 µg/ml
Lane 2 : Anti-ASK1 antibody (ab62575) at 1 µg/ml
Lane 3 : Anti-ASK1 antibody (ab62575) at 2 µg/ml
Lane 1 : SW1353 cell lysate
Lane 2 : SW1353 cell lysate
Lane 3 : SW1353 cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size : 155 kDa
Observed band size : 155 kDa -
Immunocytochemistry/ Immunofluorescence-Anti-ASK1 antibody(ab62575)Immunofluorescence of ASK1 in 3T3 cells using ab62575 at 20 ug/ml.
References for Anti-ASK1 antibody (ab62575)
ab62575 has not yet been referenced specifically in any publications.
