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Overview

  • Product nameAnti-D-glucosamine antibody
  • Description
    Rabbit polyclonal to D-glucosamine
  • SpecificityFixed tissue cross-reactivity tested with known targets at recommended dilution. No measurable glutaraldehyde-fixed tissue cross-reactivity (<1:1000) against any free D or L amino acid. The IgG is highly specifc for glucosamine (L/D differences not determined yet). As most other pentoses are unfixable (lacking primary amino groups), this antibody cannot detect them in fixed tissues.
  • Tested applicationsICC more details
  • Species reactivity
    Not applciable.
  • Immunogen

    D-glucosamine cross-linked to purifed fraction V bovine serum albumin with glutaraldehyde.

Properties

Applications

Our Abpromise guarantee covers the use of ab62666 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC
  • Application notesICC: 1/100.
    Endogenous content mapping by LM and EM immunocytochemistry. True dilution at user dilution: 1/2000. Optimal fixation: 0.1-2.5% glutaraldehyde,1% formaldehyde using HPI (High Performance Immunocytochemistry). Minimum glutaraldehyde: 0.05% using EHPI (Enhanced HPI) with 4% formaldehyde.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • RelevanceD-glucosamine, or glucosamine-6-phosphate is the biochemical precursor of all nitrogen-containing sugars. It is synthesized from fructose-6-phosphate and glutamine as the first step of the hexosamine biosynthesis pathway. The end-product of this pathway is UDP-N-acetylglucosamine (UDP-GlcNAc), which is then used for making glycosaminoglycans, proteoglycans, and glycolipids.
    • Alternative names
        Glucosamine 6 phosphate antibody

    References for Anti-D-glucosamine antibody (ab62666)

    ab62666 has not yet been referenced specifically in any publications.

    Publishing research using ab62666 ? Please let us know so that we can cite the reference in this datasheet

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