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Overview

  • Product nameZIP Kinase protein (Active)See all ZIP Kinase proteins and peptides ...
  • Protein descriptionRecombinant full length protein (Human), expressed by baculovirus in Sf9 insect cells using an N-terminal enzyme tag.
  • Expression hostBaculovirus infected sf9 cells

    • Properties

  • Purification notesThe purity was determined to be >80% by densitometry.
  • Biological activitySpecific activity: 37 nmol/min/mg.
  • FormLiquid
  • Storage instructionsShipped on dry ice. Upon delivery aliquot and store at -80ºC. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 25% Glycerol, 50mM Tris HCl, 150mM Sodium chloride, 0.25mM DTT, 0.1mM EGTA, 0.1mM EDTA, 0.1mM PMSF, pH 7.5
  • Concentration information loading...
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab63192 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE
    Functional Studies
  • Application notesKinase activity assay: Use at an assay dependent dilution.
    SDS-PAGE: Use at an assay dependent dilution.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Protein info

    • Alternative names
        DAP kinase 3DAP like kinaseDAP-like kinase
        Dapk 3Dapk3DAPK3_HUMANDeath associated kinase 3Death associated protein kinase 3Death-associated protein kinase 3DlkEC 2.7.11.1FLJ36473MYPT1 kinaseZIPZIP kinaseZIP kinase isoformZIP-kinaseZIPKzipper-interacting protein kinase
      see all
  • FunctionSerine/threonine kinase which acts as a positive regulator of apoptosis. Phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. Regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. Involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection.
  • Sequence similaritiesBelongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. DAP kinase subfamily.
    Contains 1 protein kinase domain.
  • Post-translational
    modifications    Ubiquitinated. Ubiquitination mediated by the UBE2D3 E3 ligase does not lead to proteasomal degradation, but influences promyelocytic leukemia protein nuclear bodies (PML-NBs) formation in the nucleus.
    Autophosphorylated. Phosphorylated by ROCK1.
  • Cellular localizationNucleus. Cytoplasm. Nucleus > PML body. Relocates to the cytoplasm on binding PAWR where the complex appears to interact with actin filaments (By similarity). Localizes to promyelocytic leukemia protein nuclear bodies (PML-NBs). Associates to centromeres from prophase to anaphase.

    • Target information above from: UniProt accession O43293 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    ZIP Kinase protein (Active) images

    • Functional Studies - ZIP Kinase protein (Active) (ab63192)
      Functional Studies - ZIP Kinase protein (Active) (ab63192)
      Sample Kinase Activity Plot.
    • SDS-PAGE - ZIP Kinase protein (Active) (ab63192)
      SDS-PAGE - ZIP Kinase protein (Active) (ab63192)
      ab63192 on SDS-PAGE. Molecular Weight: ~79 kDa.

    References for ZIP Kinase protein (Active) (ab63192)

    ab63192 has not yet been referenced specifically in any publications.

    Publishing research using ab63192 ? Please let us know so that we can cite the reference in this datasheet

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