Anti-Angiopoietin 2 antibody (ab64015)

Overview

• Product nameAnti-Angiopoietin 2 antibodySee all Angiopoietin 2 primary antibodies ...
• Description
  Rabbit polyclonal to Angiopoietin 2
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Chicken, Cow, Dog, Pig, Rhesus monkey
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 450 to the C-terminus of Human Angiopoietin 2.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab99482.)

• Positive controlThis antibogy gave a positive signal in the following human tissue lysates: Ovary; Cervix; Placenta; Lung.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cardiovascular
  • Angiogenesis
  • Growth Factors
  • Angiopoietin

Applications

Target

• FunctionCan induce tyrosine phosphorylation of TIE2. Binds to TIE2 receptor and counteracts blood vessel maturation/stability mediated by angiopoietin-1. Its function may be context-dependent. In the absence of angiogenic inducers, such as VEGF, ANG2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
• Sequence similaritiesContains 1 fibrinogen C-terminal domain.
• DomainThe Fibrinogen C-terminal domain mediates interaction with the TEK/TIE2 receptor.
• Cellular localizationSecreted.

Target information above from: UniProt accession O15123 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 282141 Cow
  • Entrez Gene: 607616 Dog
  • Entrez Gene: 285 Human
  • Entrez Gene: 11601 Mouse
  • Entrez Gene: 396730 Pig
  • Entrez Gene: 89805 Rat
  • Omim: 601922 Human
  • SwissProt: O77802 Cow

  • SwissProt: A0A8J8 Dog
  • SwissProt: O15123 Human
  • SwissProt: O35608 Mouse
  • SwissProt: Q9BDY7 Pig
  • SwissProt: O35462 Rat
  • Unigene: 583870 Human
  • Unigene: 439874 Mouse
  • Unigene: 138360 Rat

  see all

• Alternative names
  AGPT 2 antibodyAgpt2 antibodyANG 2 antibody

  ANG-2 antibodyANG2 antibodyAngiopoietin 2a antibodyAngiopoietin 2B antibodyAngiopoietin-2 antibodyAngiopoietin2 antibodyANGP2_HUMAN antibodyANGPT 2 antibodyANGPT2 antibodyTie2 ligand antibody

  see all

Anti-Angiopoietin 2 antibody images

• 

  Western blot - Angiopoietin 2 antibody (ab64015)
  All lanes : Anti-Angiopoietin 2 antibody (ab64015) at 1 µg/ml
  
  Lane 1 : Ovary (Human) Tissue Lysate - adult normal tissue (ab30222)
  Lane 2 : Cervix (Human) Whole Cell Lysate - adult normal tissue (ab29226)
  Lane 3 : Placenta (Human) Tissue Lysate - adult normal tissue (ab29745)
  Lane 4 : Lung (Human) Tissue Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 57 kDa
  Observed band size : 57 kDa
  Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 20 minutes