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Rabbit specific HRP/AEC (ABC) Detection IHC Kit (ab64260)

Q&A (3)

Overview

  • Product nameRabbit specific HRP/AEC (ABC) Detection IHC KitSee all HRP/AEC Detection Kit kits ...
  • Product overview

    The reagents in this kit constitute a labeled streptavidin-biotin immunoenzymatic antigen detection system. This technique involves the sequential incubation of the specimen with an unconjugated primary antibody specific to the target antigen, a biotinylated secondary antibody which reacts with the primary antibody, enzyme-labeled streptavidin, and substrate-chromogen.

  • Notes

    Contains hydrogen peroxide and Sodium azide.

  • Tested applicationsIHC-P more details

Properties

  • Storage instructionsStore at +4°C. Please refer to protocols.
  • Components 15 ml
    AEC Single Solution 1 x 15ml
    Biotinylated goat anti-rabbit IgG(H+L) 1 x 15ml
    Hydrogen Peroxide Block 1 x 15ml
    Protein block 1 x 15ml
    Streptavidin Peroxidase 1 x 15ml
  • Research Areas
  • RelevanceAn HRP/AEC Detection Kit is a labeled immunoenzymatic antigen detection system. Horseradish peroxidase (HRP) readily combines with hydrogen peroxide (H2O2) and the resultant complex can oxidize a wide variety of chromogenic substrates. AEC is 3-amino-9-ethyl-carbazole, a chromogenic substrate.
    • Alternative names
        carbazoleHorseradish Peroxidase

    Applications

    Our Abpromise guarantee covers the use of ab64260 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    IHC-P IHC-P

    Rabbit specific HRP/AEC (ABC) Detection IHC Kit images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti Rabbit HRP/AEC Detection Kit (ab64260)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti Rabbit HRP/AEC Detection Kit (ab64260)
      Human tonsil stained with anti-CD3 antibody using Anti Rabbit HRP/AEC Detection Kit (ab64260)

    References for Rabbit specific HRP/AEC (ABC) Detection IHC Kit (ab64260)

    ab64260 has not yet been referenced specifically in any publications.

    Publishing research using ab64260 ? Please let us know so that we can cite the reference in this datasheet

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    Submit a Question for ab64260

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    One of our customers wants to use ab93705 kit for IHC on Mouse samples.
    The secondary antibody included with the kit is anti Mouse/Rabbit, I'm concerned it might react with the mouse tissue and result in unspecific staining.
    Is the suitable for ...

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    Our ab93705 (Mouse and Rabbit Specific HRP/AEC (ABC) Detection IHC Kit) is not suitable for use on mouse samples. The secondary antibody is a cocktail of biotinylated anti-mouse and anti-rabbit antibodies and the anti-mouse antibodies will react...

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    xxxx;

    As I told you on the phone, I am planning on performing IHC on fresh PBMC samples from sheep.

    Cells will be isolated from whole blood using a density gradient and will be placed on glass slides using a cytocentrifuge. At this p...

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    Thank you for replying with that information.

    As I mentioned over the phone, I have spoken to the source of these kits and we do not have any experimental data using either the ACE or DABdetection kits (ab64260, ab64261, ab80437 and ab94361)for ...

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    What is the protein block?

    Thank you for contacting us. I can confirm the protein block has the following composition: PBS solution, pH 7.6 0.5% BSA 0.5% casein Less than 0.1% sodium azide I hope this information helps. Please do not hesitate to contact me should ...

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"