Anti-UBF1 antibody (ab65059)

Overview

• Product nameAnti-UBF1 antibodySee all UBF1 primary antibodies ...
• Description
  Rabbit polyclonal to UBF1
• Tested applicationsWB, IHC-P, ELISA more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat
• Immunogen

  Synthetic peptide derived from an internal sequence of human UBF1.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Polymerase associated factors
  • Pol I Transcription

Applications

Target

• FunctionRecognizes the ribosomal RNA gene promoter and activates transcription mediated by RNA polymerase I through cooperative interactions with the transcription factor SL1/TIF-IB complex. It binds specifically to the upstream control element.
• Sequence similaritiesContains 6 HMG box DNA-binding domains.
• Post-translational
  modificationsPhosphorylated and activated by PIK3CA.
• Cellular localizationNucleus > nucleolus.

Target information above from: UniProt accession P17480 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7343 Human
  • Entrez Gene: 7343 Human
  • Entrez Gene: 21429 Mouse
  • Entrez Gene: 25574 Rat
  • Omim: 600673 Human
  • SwissProt: P17480 Human
  • SwissProt: P17480 Human
  • SwissProt: P25976 Mouse

  • SwissProt: P25977 Rat
  • Unigene: 89781 Human
  • Unigene: 2845 Mouse
  • Unigene: 22469 Rat

  see all

• Alternative names
  Autoantigen NOR-90 antibodyNOR 90 antibodyNucleolar transcription factor 1 antibody

  UBF 1 antibodyUBF antibodyUBF-1 antibodyUBF1_HUMAN antibodyUBTF antibodyUpstream binding factor 1 antibodyupstream binding transcription factor, RNA polymerase I antibodyUpstream-binding factor 1 antibody

  see all

Anti-UBF1 antibody images

• 

  Western blot - UBF1 antibody (ab65059)
  All lanes : Anti-UBF1 antibody (ab65059) at 1/500 dilution
  
  Lane 1 : Extracts from HeLa cells at 5-30ug
  Lane 2 : Extracts from Jurkat cells at 5-30ug
  Lane 3 : Extracts from Jurkat cells at 5-30ug with immunizing peptide at 5-10ug
  
  
  Predicted band size : 89 kDa
  Observed band size : 89 kDa
  Additional bands at : 45 kDa,70 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - UBF1 antibody (ab65059)
  Immunohistochemistry analysis of paraffin-embedded human brain tissue using ab65059 at a dilution of 1/50-1/100. Left hand panel: without immunizing peptide. Right hand panel: with immunizing peptide.