Anti-Nav1.7 antibody (ab65167)
Key features and details
- Rabbit polyclonal to Nav1.7
- Suitable for: IHC-Fr, IHC-P, IHC-FoFr, ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-Nav1.7 antibody
See all Nav1.7 primary antibodies -
Description
Rabbit polyclonal to Nav1.7 -
Host species
Rabbit -
Tested applications
Suitable for: IHC-Fr, IHC-P, IHC-FoFr, ICC/IF, WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat -
Immunogen
Synthetic peptide within Human Nav1.7 aa 1000-1100. The exact sequence is proprietary.
(Peptide available asab192016) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
Constituent: Whole serum -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab65167 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-Fr |
Use at an assay dependent concentration.
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IHC-P | (1) |
Use at an assay dependent concentration.
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IHC-FoFr |
Use at an assay dependent concentration. PubMed: 21441906
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ICC/IF | (1) |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration.
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Notes |
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IHC-Fr
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
IHC-FoFr
Use at an assay dependent concentration. PubMed: 21441906 |
ICC/IF
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Target
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Function
Mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient (PubMed:7720699, PubMed:17167479, PubMed:25240195, PubMed:26680203, PubMed:15385606, PubMed:16988069, PubMed:17145499, PubMed:19369487, PubMed:24311784). It is a tetrodotoxin-sensitive Na(+) channel isoform (PubMed:7720699). Plays a role in pain mechanisms, especially in the development of inflammatory pain (PubMed:17167479, PubMed:17145499, PubMed:19369487, PubMed:24311784). -
Tissue specificity
Expressed strongly in dorsal root ganglion, with only minor levels elsewhere in the body, smooth muscle cells, MTC cell line and C-cell carcinoma. Isoform 1 is expressed preferentially in the central and peripheral nervous system. Isoform 2 is expressed preferentially in the dorsal root ganglion. -
Involvement in disease
Primary erythermalgia
Indifference to pain, congenital, autosomal recessive
Paroxysmal extreme pain disorder
Generalized epilepsy with febrile seizures plus 7
Febrile seizures, familial, 3B -
Sequence similarities
Belongs to the sodium channel (TC 1.A.1.10) family. Nav1.7/SCN9A subfamily.
Contains 1 IQ domain. -
Domain
The sequence contains 4 internal repeats, each with 5 hydrophobic segments (S1,S2,S3,S5,S6) and one positively charged segment (S4). Segments S4 are probably the voltage-sensors and are characterized by a series of positively charged amino acids at every third position. -
Post-translational
modificationsPhosphorylation at Ser-1490 by PKC in a highly conserved cytoplasmic loop increases peak sodium currents.
Ubiquitinated by NEDD4L; which may promote its endocytosis. Does not seem to be ubiquitinated by NEDD4. -
Cellular localization
Cell membrane. Cell projection. In neurite terminals. - Information by UniProt
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Database links
- Entrez Gene: 6335 Human
- Entrez Gene: 20274 Mouse
- Entrez Gene: 78956 Rat
- Omim: 603415 Human
- SwissProt: Q15858 Human
- SwissProt: Q62205 Mouse
- SwissProt: O08562 Rat
- Unigene: 439145 Human
see all -
Alternative names
- ETHA antibody
- GEFSP7 antibody
- hNE Na antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab65167 has been referenced in 8 publications.
- Kwon M et al. Inhibition of the Nav1.7 Channel in the Trigeminal Ganglion Relieves Pulpitis Inflammatory Pain. Front Pharmacol 12:759730 (2021). PubMed: 34955831
- Atmaramani RR et al. Conserved Expression of Nav1.7 and Nav1.8 Contribute to the Spontaneous and Thermally Evoked Excitability in IL-6 and NGF-Sensitized Adult Dorsal Root Ganglion Neurons In Vitro. Bioengineering (Basel) 7:N/A (2020). PubMed: 32429423
- Hulme AJ et al. Molecular and Functional Characterization of Neurogenin-2 Induced Human Sensory Neurons. Front Cell Neurosci 14:600895 (2020). PubMed: 33362470
- Sun W et al. Inhibition of lncRNA X inactivate-specific transcript ameliorates inflammatory pain by suppressing satellite glial cell activation and inflammation by acting as a sponge of miR-146a to inhibit Nav 1.7. J Cell Biochem 119:9888-9898 (2018). PubMed: 30129228
- Cai YY et al. Effects of SCN9A gene modification on Na+ channel and the expression of nerve growth factor in a rat model of diarrhea-predominant irritable bowel syndrome. Mol Med Rep 17:1839-1846 (2018). PubMed: 29138838
- Rice FL et al. Anatomy and immunochemical characterization of the non-arterial peptidergic diffuse dural innervation of the rat and Rhesus monkey: Implications for functional regulation and treatment in migraine. Cephalalgia 37:1350-1372 (2017). PubMed: 27852962
- Henningsen K et al. Candidate hippocampal biomarkers of susceptibility and resilience to stress in a rat model of depression. Mol Cell Proteomics 11:M111.016428 (2012). IHC-FoFr ; Rat . PubMed: 22311638
- Weiss J et al. Loss-of-function mutations in sodium channel Nav1.7 cause anosmia. Nature 472:186-90 (2011). IHC-FoFr ; Mouse . PubMed: 21441906