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Caspase 9 (active) Red Staining Kit (ab65619)

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Overview

  • Product nameCaspase 9 (active) Red Staining KitSee all Caspase 9 kits ...
  • Tests
    100 x 1 assay
  • Sample type
    Adherent cells, Suspension cells
  • Assay typeEnzyme activity
  • Assay time
    2h 00m
  • Product overview

    Abcam's Caspase 9 (active) Red Staining Kit provides a convenient means for detecting activated caspase 9 in living cells. The assay utilizes the caspase 9 inhibitor LEHD-FMK conjugated to sulfo-rhodamine (Red-LEHD-FMK) as the fluorescent in situ marker. Red-LEHD-FMK is cell permeable, nontoxic, and irreversibly binds to activated caspase 9 in apoptotic cells.
    Visit our FAQs page for tips and troubleshooting.

  • Notes

    Activation of caspases plays a central role in apoptosis.

  • Tested applicationsFluorescence Microscopy, Functional Studies, Flow Cyt more details

Properties

  • RelevanceCaspases are cysteine proteases, expressed as inactive precursors, that mediate apoptosis by proteolysis of specific substrates. Caspases have the ability to cleave after aspartic acid residues. There are two classes of caspases involved in apoptosis; initiators (activation by receptor cluster) and effectors (activation by mitochondrial permeability transition). Proapoptotic signals autocatalytically activate initiator caspases, such as Caspase 8 and Caspase 9. Activated initiator caspases then process effector caspases, such as Caspase 3 and Caspase 7, which in turn cause cell collapse. Caspase 9 (also known as ICE like apoptotic protease 6 (ICE LAP6), apoptotic protease Mch6, and apoptotic protease activating factor 3 (Apaf3)) is a member of the peptidase family C14 that contains a CARD domain. It is active as a heterotetramer and has been reported to have two isoforms. ProCaspase 9 is approximately 47 kD. It is present in the cytosol and, upon activation, translocates to the mitochondria. Caspase 9 is involved in the caspase activation cascade responsible for apoptosis execution and cleaves/activates Caspase 3 and Caspase 6. It becomes activated when recruited to the Apaf1/cytochrome c complex.
    • Alternative names
        APAF3CASP9Caspase 9
        ICE LAP6MCH6
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    Applications

    Our Abpromise guarantee covers the use of ab65619 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    Fluorescence Microscopy Fluorescence Microscopy
    Functional Studies FuncS
    Flow Cyt Flow Cyt

    Protocols

    References for Caspase 9 (active) Red Staining Kit (ab65619)

    ab65619 has not yet been referenced specifically in any publications.

    Publishing research using ab65619 ? Please let us know so that we can cite the reference in this datasheet

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"