Anti-Archaemetzincin 2 antibody - Catalytic domain (ab65885)

Overview

• Product nameAnti-Archaemetzincin 2 antibody - Catalytic domainSee all Archaemetzincin 2 primary antibodies ...
• Description
  Rabbit polyclonal to Archaemetzincin 2 - Catalytic domain
• Specificityab65885 recognizes all of the forms of Archaemetzincin 2. We have a range of domain specific antibodies for this target. For a full list please see all Archaemetzincin 2 antibodies.
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  
• Immunogen

  A synthetic peptide based on the catalytic domain of human Archaemetzincin 2.

• Positive controlHFL-1 lysate treated with TPA, IL1 beta, beta-estradiol or Testosterone, or serum free.

Properties

• FormLiquid
• Storage instructionsStore at -20°C. Stable for 12 months at -20°C
• Storage bufferPreservative: 0.05% Sodium Azide
  Constituents: 50% Glycerol, PBS, 500mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteolytic enzymes
  • Other proteases

Applications

Target

• FunctionZinc metalloprotease. Exhibits activity against angiotensin-3 in vitro. Does not hydrolyze either neurogranin or angiotensin-2.
• Tissue specificityPredominantly expressed in heart and testis. Also expressed at lower levels in kidney, liver, pancreas, lung, brain and placenta. Expressed in fetal tissues such as kidney, liver, lung and brain.
• Sequence similaritiesBelongs to the peptidase M54 family.

Target information above from: UniProt accession Q86W34 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 51321 Human
  • SwissProt: Q86W34 Human
  • Unigene: 293560 Human

• Alternative names
  AMZ2 antibodyAMZ2_HUMAN antibodyArchaemetzincin 2 antibody

  Archaemetzincin-2 antibodyArcheobacterial metalloproteinase like protein 2 antibodyArcheobacterial metalloproteinase-like protein 2 antibody

  see all

Anti-Archaemetzincin 2 antibody - Catalytic domain images

• 

  Western blot - Archaemetzincin 2 antibody (ab65885)
  All lanes : Anti-Archaemetzincin 2 antibody - Catalytic domain (ab65885) at 1/5000 dilution
  
  Lane 1 : HFL-1 lysate (1/4 dilution) - Serum Free
  Lane 2 : HFL-1 lysate (1/4 dilution) treated with TPA
  Lane 3 : HFL-1 lysate (1/4 dilution) treated with IL1 beta
  Lane 4 : HFL-1 lysate (1/4 dilution) treated with beta-estradiol
  Lane 5 : HFL-1 lysate (1/4 dilution) treated with testosterone
  
  Lysates/proteins at 35 µl per lane.
  
  
  Predicted band size : 41 kDa
  Observed band size : 41 kDa
  Additional bands at : 110 kDa,45 kDa,49 kDa (possible isoform),66 kDa. We are unsure as to the identity of these extra bands.