Overview
- Product nameAnti-AAK1 antibodySee all AAK1 primary antibodies ...
- DescriptionRabbit polyclonal to AAK1
- SpecificityThis antibody detects endogenous levels of total AAK1 protein.
- Tested applicationsELISA, WB more details
- Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat - Immunogen
A synthetic peptide derived from the internal region of human AAK1
- Positive controlExtracts from COS7 cells
Properties
- FormLiquid
- Storage instructionsStore at -20°C. Stable for 12 months at -20°C
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 50% Glycerol, PBS, 150mM Sodium chloride, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesThe antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab66005 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | ELISA: 1/20000. |
| WB | WB: 1/500 - 1/1000. Detects a band of approximately 94, 75 kDa (predicted molecular weight: 94 kDa). |
Target
- FunctionPhosphorylates the AP2M1/mu2 subunit of the adaptor protein complex 2 (AP-2). May play a role in regulating aspects of clathrin-mediated endocytosis.
- Sequence similaritiesBelongs to the protein kinase superfamily. Ser/Thr protein kinase family.
Contains 1 protein kinase domain. - Post-translational
modificationsAutophosphorylated. - Cellular localizationMembrane. Cell membrane. Membrane > clathrin-coated pit. Active when found in clathrin-coated pits at the plasma membrane.
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Database links
- Entrez Gene: 22848 Human
- Entrez Gene: 22848 Human
- Entrez Gene: 22848 Human
- Entrez Gene: 269774 Mouse
- Entrez Gene: 500244 Rat
- SwissProt: Q2M2I8 Human
- SwissProt: Q2M2I8 Human
- SwissProt: Q2M2I8 Human
- SwissProt: Q3UHJ0 Mouse
- SwissProt: P0C1X8 Rat
- Unigene: 468878 Human
- Unigene: 221038 Mouse
- Unigene: 203307 Rat
see all
Target information above from: UniProt accession
Q2M2I8
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- AAK 1 antibodyAak1 antibodyAAK1_HUMAN antibody
- Adaptor associated kinase 1 antibodyAdaptor-associated kinase 1 antibodyAP2 associated kinase 1 antibodyAP2 associated protein kinase 1 antibodyAP2-associated protein kinase 1 antibodyDKFZp686F03202 antibodyDKFZp686K16132 antibodyFLJ23712 antibodyFLJ25931 antibodyFLJ31060 antibodyFLJ42882 antibodyFLJ45252 antibodyKIAA1048 antibodyMGC138170 antibodyMGC164568 antibodyMGC164570 antibody
see all
Anti-AAK1 antibody images
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Western blot - AAK1 antibody (ab66005)All lanes : Anti-AAK1 antibody (ab66005) at 1/500 dilution
Lane 1 : Extracts from COS7 cells
Lane 2 : Extracts from COS7 cells, plus 5µg immunizing peptide
Lysates/proteins at 5 µg per lane.
Predicted band size : 94 kDa
Observed band size : 94 kDa
Additional bands at : 75 kDa. We are unsure as to the identity of these extra bands.
References for Anti-AAK1 antibody (ab66005)
ab66005 has not yet been referenced specifically in any publications.
