Overview
- Product nameAnti-TGF beta antibodySee all TGF beta primary antibodies ...
- DescriptionRabbit polyclonal to TGF beta
- Tested applicationsWB, IP, ELISA, IHC-Fr, ICC/IF, IHC-P, Flow Cyt more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
A synthetic peptide (17 aa) derived from C terminal human TGF beta 3 protein. (Peptide available as ab119811.)
- Positive controlBreast carcinomas (MCF-7), Human Placenta, Intestine.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Proclin
Constituents: 1% BSA, PBS, pH 7.2 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
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Research Areas
Applications
Our Abpromise guarantee covers the use of ab66043 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 0.1 - 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).Can be blocked with TGF beta peptide (ab119811). Epitope Retrieval is recommend as follows: 10 minutes 95 degrees followed by 20 minutes for cool down. |
| IP | IP: Use a concentration of 2 - 5 µg/ml. |
| ELISA | ELISA: Use a concentration of 0.01 - 0.1 µg/ml. |
| IHC-Fr | IHC-Fr: Use at an assay dependent concentration. |
| ICC/IF | ICC/IF: Use a concentration of 1 µg/ml. |
| IHC-P | IHC-P: Use a concentration of 2 - 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
| Flow Cyt | Flow Cyt: Use at an assay dependent concentration. |
Target
- FunctionMultifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts.
- Tissue specificityHighly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA). Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
- Involvement in diseaseDefects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MIM:131300]; also known as progressive diaphyseal dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
- Sequence similaritiesBelongs to the TGF-beta family.
- Post-translational
modificationsGlycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it inactive. - Cellular localizationSecreted > extracellular space > extracellular matrix.
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Database links
- Entrez Gene: 7042 Human
- Entrez Gene: 7043 Human
- Entrez Gene: 7040 Human
- Entrez Gene: 21808 Mouse
- Entrez Gene: 21809 Mouse
- Entrez Gene: 21803 Mouse
- Entrez Gene: 81809 Rat
- Entrez Gene: 25717 Rat
- Entrez Gene: 59086 Rat
- Omim: 190180 Human
- Omim: 190220 Human
- SwissProt: P61812 Human
- SwissProt: P10600 Human
- SwissProt: P01137 Human
- SwissProt: P27090 Mouse
- SwissProt: P17125 Mouse
- SwissProt: P04202 Mouse
- SwissProt: Q07257 Rat
- SwissProt: Q07258 Rat
- SwissProt: P17246 Rat
- Unigene: 645227 Human
- Unigene: 248380 Mouse
- Unigene: 40136 Rat
see all
Target information above from: UniProt accession
P01137
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- ARVD antibodyBSC 1 cell growth inhibitor antibodyCED antibody
- Cetermin antibodyDiaphyseal dysplasia 1 progressive antibodyDPD 1 antibodyDPD1 antibodyG TSF antibodyGlioblastoma derived T cell suppressor factor antibodyLAP antibodyLatency-associated peptide antibodyPolyergin antibodyTGF beta 1 antibodyTGF beta 1 protein antibodyTGF beta 3 antibodyTGF beta antibodyTGF beta1 antibodyTGF beta2 antibodyTGF beta3 antibodyTGF-beta-1 antibodyTGFB 1 antibodyTGFB antibodyTGFB1 antibodyTGFB1_HUMAN antibodyTGFB2 antibodyTGFB3 antibodyTGFbeta antibodyTransforming growth factor antibodyTransforming growth factor beta 1 antibodyTransforming growth factor beta 2 antibodyTransforming growth factor beta 3 antibodyTransforming Growth Factor beta antibody
see all
Anti-TGF beta antibody images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TGF beta antibody (ab66043)Human placenta stained with Anti-TGF beta antibody (ab66043) at 1/100. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - TGF beta antibody (ab66043)This image is courtesy of an Abreview submitted by Ross Benikab66043 staining TGF beta in Rat liver tissue sections by Immunohistochemistry (IHC-P - formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 0.25% serum for 15 minutes at 25°C ; antigen retrieval was by heat mediation in citrate (pH 6.0) buffer. Samples were incubated with primary antibody (1/50 in serum free antibody diluent) for 18 hours at 4°C. An undiluted HRP-conjugated Goat polymer was used as the secondary antibody. -
Western blot - TGF beta antibody (ab66043)Anti-TGF beta antibody (ab66043) at 1/500 dilution (for 18 hours at 4°C) + Whole tissue lysate of mouse brain at 20 µg
Secondary
An HRP-conjugated Goat polyclonal at 1/10000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 13 kDa
Observed band size : 13 kDa
Exposure time : 90 secondsThis image is courtesy of an anonymous Abreview
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Western blot - TGF beta antibody (ab66043)Anti-TGF beta antibody (ab66043) at 1/500 dilution + whole tissue lysate prepared from rat intestine at 15 µg
Secondary
Goat anti-rabbit conjugated to HRP at 1/5000 dilution
developed using the ECL technique
Predicted band size : 13 kDa
Observed band size : 13 kDa
Exposure time : 2 minutesImage courtesy of an anonymous Abreview.
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Immunocytochemistry/ Immunofluorescence - Anti-TGF beta antibody (ab66043)ICC/IF image of ab66043 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab66043, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-TGF beta antibody (ab66043)
This product has been referenced in:
- Xie XL et al. 2-Amino-3-methylimidazo[4,5-f]quinoline (IQ) promotes mouse hepatocarcinogenesis by activating transforming growth factor-ß and Wnt/ß-catenin signaling pathways. Toxicol Sci 125:392-400 (2012). Read more (PubMed: 22094457) »
- Becker LE et al. Effect of paricalcitol and calcitriol on aortic wall remodeling in uninephrectomized ApoE knockout mice. Am J Physiol Renal Physiol 300:F772-82 (2011). IHC-P ; Mouse . Read more (PubMed: 21159735) »
