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Overview

  • Product nameAnti-IL6 antibodySee all IL6 primary antibodies ...
  • Description
    Mouse monoclonal to IL6
  • Tested applicationsELISA, WB more details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human) with tag.

  • Positive controlIL6 transfected 293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab66231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ELISA ELISA: Use at an assay dependent dilution.
WB WB: Use a concentration of 1 - 5 µg/ml. Detects a band of approximately 27 kDa (predicted molecular weight: 24 kDa).

Target

  • FunctionCytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance.
  • Involvement in diseaseGenetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
    Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men.
  • Sequence similaritiesBelongs to the IL-6 superfamily.
  • Post-translational
    modifications    N- and O-glycosylated.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P05231 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    • Alternative names
        Interleukin BSF 2 antibodyB cell differentiation factor antibodyB cell stimulatory factor 2 antibody
        B-cell stimulatory factor 2 antibodyBSF 2 antibodyBSF-2 antibodyBSF2 antibodyCDF antibodyCTL differentiation factor antibodyCytotoxic T cell differentiation factor antibodyHepatocyte stimulating factor antibodyHGF antibodyHPGF antibodyHSF antibodyHybridoma growth factor antibodyHybridoma growth factor Interferon beta-2 antibodyHybridoma plasmacytoma growth factor antibodyIFN-beta-2 antibodyIFNB2 antibodyIL 6 antibodyIL-6 antibodyIL6 antibodyIL6 protein antibodyIL6_HUMAN antibodyInterferon beta 2 antibodyInterferon beta-2 antibodyInterleukin 6 antibodyInterleukin 6 (interferon beta 2) antibodyInterleukin-6 antibodyInterleukin6 antibody
      see all

    Anti-IL6 antibody images

    • Western blot - IL6 antibody (ab66231)
      Western blot - IL6 antibody (ab66231)
      All lanes : Anti-IL6 antibody (ab66231) at 1 µg/ml

      Lane 1 : IL6 transfected 293T cell lysate
      Lane 2 : Non-transfected 293T cell lysate.

      Lysates/proteins at 50 µg per lane.

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution

      Predicted band size : 24 kDa
      Observed band size : 27 kDa (why is the actual band size different from the predicted?)
      Additional bands at : 21 kDa. We are unsure as to the identity of these extra bands.

    References for Anti-IL6 antibody (ab66231)

    This product has been referenced in:
    • Feairheller DL  et al. Racial differences in oxidative stress and inflammation: in vitro and in vivo. Clin Transl Sci 4:32-7 (2011). WB ; Human . Read more (PubMed: 21348953) »

    See 1 Publication for this product

    Publishing research using ab66231 ? Please let us know so that we can cite the reference in this datasheet

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