Anti-GFP antibody (FITC) (ab6662)

Overview

• Product nameAnti-GFP antibody (FITC)See all GFP primary antibodies ...
• Description
  Goat polyclonal to GFP (FITC)
• ConjugationFITC
• Conjugation notesFluorescein isothiocyanate (FITC) (MW 390 daltons) Absorption Wavelength: 495 nm Emission Wavelength: 528 nm Fluorochrome/Protein Ratio: 3.5 moles FITC per mole of Goat IgG
• SpecificityThis GFP antibody will cross react with YFP, CFP, eGFP, but not RFP.
• Tested applicationsIHC-FoFr, IHC-P, ELISA, IHC-Fr, WB, ICC/IF more details
• Immunogen

  Green Fluorescent Protein (GFP) fusion protein corresponding to the full length amino acid sequence (246aa) derived from the jellyfish Aequorea victoria.

• General notes

  
  

  Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation. Fluorescein conjugated anti-GFP was assayed by immunofluorescence microscopy on prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.01% Sodium Azide
  Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Green Fluorescent Protein (Aequorea victoria) coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
• Primary antibody notes Designed to detect GFP and its variants in ELISA (sandwich or capture), immunoblotting and immunoprecipitation. Fluorescein conjugated anti-GFP was assayed by immunofluorescence microscopy on prokaryotic (E.coli) and eukaryotic (CHO cells) expression systems and was shown to detect GFP containing inserts. Significant amplification of signal was detected using fluorochrome conjugated anti-GFP relative to the fluorescence of GFP alone.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Tags & Cell Markers
  • Fusion / Marker Proteins
  • GFP

Applications

Target

• RelevanceGreen fluorescent protein (GFP) is a 27 kDa protein derived from the jellyfish Aequorea victoria, which emits green light (emission peak at a wavelength of 509 nm) when excited by blue light (excitation peak at a wavelength of 395 nm). GFP has become an invaluable tool in cell biology research, since its intrinsic fluorescence can be visualized in living cells. GFP fluorescence is stable under fixation conditions and suitable for a variety of applications. GFP has been widely used as a reporter for gene expression, enabling researchers to visualize and localize GFP-tagged proteins within living cells without the need for chemical staining. Other applications of GFP include assessment of protein protein interactions through the yeast two hybrid system and measurement of distance between proteins through fluorescence energy transfer (FRET) protocols. GFP technology has considerably contributed to a greater understanding of cellular physiology. YFP differs from GFP due to a mutation at T203Y; antibodies raised against full-length GFP should also detect YFP and other variants.

• Alternative names
  GFP antibodyGreen Fluorescent Protein antibody

Anti-GFP antibody (FITC) images

• 

  Immunocytochemistry/ Immunofluorescence - GFP antibody (FITC) (ab6662)

  These pictures show confocal immunofluorescence using GFP-expressing glial cells (green) transplanted into the lesioned rat spinal cord. This was detected using ab6662 and a standard FITC filter set. Axons are labelled red by an antibody to neurofilament-200 and a rhodamine secondary antibody. The upper panel shows the centre of the transplant site at low power. Numerous GFP-positive cells can be seen mingling with axons. The lower panel shows, at high power in a single optical section, how ab6662 reveals the morphology of the transplanted cells to such an extent that their close interactions with axons are obvious - the cell depicted can be seen wrapping around a neurofilament-200 positive axon.

  These images were kindly supplied as part of the review submitted by Andrew Toft.

• 

  Immunohistochemistry (Frozen sections) - GFP antibody (FITC) (ab6662)This image is courtesy of an Abreview submitted by Dr Joshua Breunig

  ab6662 staining mouse brain tissue sections (inducible GFP reporter) by IHC-Fr. The tissue was paraformaldehyde fixed and blocked with serum and then incubated with the antibody at a 1/1000 dilution for 1 hour.

  Staining is shown in the left hand panel. The middle panel shows staining with a rabbit anti-GFP antibody and the right hand panel shows the merged images (plus DAPI). ab6662 gives no noticable background and it is found that when viewing on an epifluorescent the exposure time is significantly reduced.

  See Abreview