Anti-Ube2L3 antibody (ab66944)

Overview

• Product nameAnti-Ube2L3 antibodySee all Ube2L3 primary antibodies ...
• Description
  Rabbit polyclonal to Ube2L3
• Tested applicationsWB, ELISA, IHC-P more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Rat, Cow, Dog, Pig, Zebrafish
• Immunogen

  Synthetic peptide: SLIALVNDPQ PEHPLRADLA EEYSKDRKKF CKNAEEFTKK YGEKRPVD, corresponding to C terminal amino acids 106-155 of Human Ube2L3

  Run BLAST with Run BLAST with
• Positive controlJurkat cell lysate Human intestine tissue

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 2% Sucrose, PBS
• Concentration information loading...
• PurityProtein A purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Ubiquitin & Ubiquitin Like Modifiers
  • E2 Ubiquitin Conjugating Enzymes

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteasome / Ubiquitin
  • Ubiquitin E2s and E3s
  • Ubiquitin E2 Enzymes

Applications

Target

• FunctionAccepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'-linked polyubiquitination. Involved in the selective degradation of short-lived and abnormal proteins. Down-regulated during the S-phase it is involved in progression through the cell cycle. Regulates nuclear hormone receptors transcriptional activity. May play a role in myelopoiesis.
• Tissue specificityUbiquitous, with highest expression in testis.
• PathwayProtein modification; protein ubiquitination.
• Sequence similaritiesBelongs to the ubiquitin-conjugating enzyme family.
• Post-translational
  modificationsUbiquitinated. The alteration of UBE2L3 protein levels during the S-phase of the cell cycle is due to ubiquitin-dependent proteasomal degradation.
• Cellular localizationNucleus. Cytoplasm.

Target information above from: UniProt accession P68036 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 767894 Cow
  • Entrez Gene: 7332 Human
  • Entrez Gene: 100042355 Mouse
  • Entrez Gene: 22195 Mouse
  • Entrez Gene: 363836 Rat
  • Entrez Gene: 321943 Zebrafish
  • Omim: 603721 Human
  • SwissProt: Q3MHP1 Cow

  • SwissProt: P68036 Human
  • SwissProt: P68037 Mouse
  • Unigene: 108104 Human
  • Unigene: 603229 Human
  • Unigene: 3074 Mouse
  • Unigene: 163186 Rat

  see all

• Alternative names
  E2 F1 antibodyE2-F1 antibodyL UBC antibody

  L-UBC antibodyUB2L3_HUMAN antibodyUBCE7 antibodyUbcH7 antibodyUbcM4 antibodyUbe2l3 antibodyUbiquitin carrier protein L3 antibodyUbiquitin conjugating enzyme E2 L3 antibodyUbiquitin protein ligase L3 antibodyUbiquitin-conjugating enzyme E2 L3 antibodyUbiquitin-conjugating enzyme E2-F1 antibodyUbiquitin-protein ligase L3 antibody

  see all

Anti-Ube2L3 antibody images

• 

  Western blot - Ube2L3 antibody (ab66944)
  Anti-Ube2L3 antibody (ab66944) at 1.25 µg/ml + Jurkat cell lysate at 10 µg
  
  Secondary
  HRP conjugated anti-Rabbit IgG at 1/50000 dilution
  
  Predicted band size : 18 kDa
  Observed band size : 18 kDa
  Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Ube2L3 antibody (ab66944)
  Immunohistochemical analysis of paraffin embedded human intestine tissue labelled with ab66944 at 4-8µg/ml. Positively labelled epithelial cells of intestinal villus are indicated with arrows. Magnification: 400X.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)-Anti-Ube2L3 antibody(ab66944)
  Immunohistochemistry of Human Intestine tissue at an antibody concentration of 4-8µg/ml using anti-UBE2L3 antibody (ab66944)