Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)
Overview
- Product nameGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)See all IgG secondary antibodies ...
- Target speciesRabbit
- Tested applicationsIHC-P, WB, ELISA, Immunomicroscopy, Dot Blot, ICC/IF, IHC-Fr more details
- Immunogen
Rabbit IgG, whole molecule
Properties
- FormLiquid
- Storage instructionsStore at +4°C in the dark. Do not freeze.
- Storage bufferPreservative: 0.01% Gentamicin Sulfate
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2 -
Concentration information loading... - PurityIgG fraction
- Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads.
- Conjugation notesHorseradish Peroxidase (HRP)
- Clonality Polyclonal
- General notesMany of our customers have reported seeing brown precipitates in the vials. The brown precipitates are very common with HRP conjugated antibodies; we suggest vortexing the vial and using this antibody as normal. Our customer’s feedback says the antibody worked great. If in case the antibody fails to give results then please contact our Scientific Support team for assistance.
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab6721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | IHC-P: 1/1000. |
| WB | WB: 1/2000 - 1/20000. Suggested working dilution of 1/3000 (see PMID: 17222046). In addition, found to work at 1/20000 (see PMID: 16936283).Working dilutions are highlighted in the table below. Please note that the antibody can be diluted to 1:48,000 to 1:207,000 in many instances. |
| ELISA | ELISA: 1/120000. |
| Immunomicroscopy | IM: Use at an assay dependent dilution. |
| Dot Blot | Dot: Use at an assay dependent dilution. |
| ICC/IF | ICC/IF: 1/1000 - 1/5000. |
| IHC-Fr | IHC-Fr: 1/1000. |
Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) images
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Western blot - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)Ab6721 was used with the primary antibody ab8227 in WB. See the review on ab8227. -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)Ab6721 was used at dilution 1/100 with the primary antibody ab11370 in IHC-P. See the review on ab11370. -
Immunohistochemistry (Frozen sections) - Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)Ab6721 was used at dilution 1/100 with the primary antibody ab35604 in IHC-Fr. See the review on ab35604.
References for Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP) (ab6721)
This product has been referenced in:
- Mancek-Keber M et al. MARCKS as a negative regulator of lipopolysaccharide signaling. J Immunol 188:3893-902 (2012). Read more (PubMed: 22427633) »
- Benneyworth MA et al. Failure of NMDA receptor hypofunction to induce a pathological reduction in PV-positive GABAergic cell markers. Neurosci Lett 488:267-71 (2011). WB . Read more (PubMed: 21094213) »
