Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab6734)
Overview
- Product nameRabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP)See all IgG secondary antibodies ...
- Target speciesRat
- Tested applicationsIHC-P, WB, ELISA, Immunomicroscopy, Dot Blot, ICC/IF, IHC-Fr more details
- Immunogen
Rat IgG whole molecule
Properties
- FormLiquid
- Storage instructionsStore at +4°C in the dark. Do not freeze.
- Storage bufferPreservative: 0.01% Gentamicin Sulfate
Constituents: 10mg/ml BSA, 0.15M Sodium chloride, 0.02M Potassium phosphate, pH 7.2 -
Concentration information loading... - PurityIgG fraction
- Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
- Conjugation notesHorseradish Peroxidase (HRP)
- Clonality Polyclonal
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab6734 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| IHC-P | IHC-P: Use at an assay dependent concentration. |
| WB | WB: 1/1000 - 1/5000. |
| ELISA | ELISA: 1/60000. |
| Immunomicroscopy | IM: Use at an assay dependent concentration. |
| Dot Blot | Dot: Use at an assay dependent concentration. |
| ICC/IF | ICC/IF: 1/500 - 1/2500. |
| IHC-Fr | IHC-Fr: Use at an assay dependent concentration. |
Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP) images
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Western blot - Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab6734)Ab6734 was used at dilution 1/5000 with the primary antibody ab33731 in WB. See the review on ab33731.
References for Rabbit polyclonal Secondary Antibody to Rat IgG - H&L (HRP) (ab6734)
This product has been referenced in:
- Epis MR et al. Regulation of Expression of Deoxyhypusine Hydroxylase (DOHH), the Enzyme That Catalyzes the Activation of eIF5A, by miR-331-3p and miR-642-5p in Prostate Cancer Cells. J Biol Chem 287:35251-9 (2012). WB . Read more (PubMed: 22908221) »
- Epis MR et al. The RNA-binding protein HuR opposes the repression of ERBB-2 gene expression by microRNA miR-331-3p in prostate cancer cells. J Biol Chem 286:41442-54 (2011). WB . Read more (PubMed: 21971048) »
