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Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (TRITC ) (ab6744)

Overview

  • Product nameRabbit polyclonal Secondary Antibody to Sheep IgG - H&L (TRITC )See all IgG secondary antibodies ...
  • Target speciesSheep
  • Tested applicationsImmunomicroscopy, Flow Cyt, ICC/IF, IHC-P, IHC-Fr, ELISA more details
  • Immunogen

    Sheep IgG whole molecule

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesTetramethylrhodamine isothiocyanate (TRITC) (Molecular Weight 444 daltons) Absorption Wavelength: 550 nm Emission Wavelength: 570 nm Fluorochrome/Protein Ratio: 2.8 moles TRITC per mole of Rabbit IgG.
  • Conjugation notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Clonality Polyclonal
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6744 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Immunomicroscopy
Flow Cyt
ICC/IF
IHC-P
IHC-Fr
ELISA
  • Application notesSuitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency
  • References for Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (TRITC ) (ab6744)

    This product has been referenced in:
    • Gunawan RC  et al. Complementary targeting of liposomes to IL-1a and TNF-a activated endothelial cells via the transient expression of VCAM1 and E-selectin. Biomaterials 32:9848-53 (2011). Read more (PubMed: 21944721) »

    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"