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Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (Biotin) (ab6746)

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Overview

  • Product nameRabbit polyclonal Secondary Antibody to Sheep IgG - H&L (Biotin)See all IgG secondary antibodies ...
  • Target speciesSheep
  • Tested applicationsWB, ELISA, Immunomicroscopy, Dot Blot, ICC/IF, IHC-P, IHC-Fr more details
  • Immunogen

    Sheep IgG whole molecule

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer0.01M Na phosphate, 0.25M NaCl, pH7.6, 0.05% Na azide, 0.01% Thimerisol, 15mg/ml BSA
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Rabbit IgG molecule
  • Conjugation notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Sheep IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities
  • Clonality Polyclonal
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6746 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/1000 - 1/5000.
ELISA ELISA: 1/140000.
Immunomicroscopy IM: Use at an assay dependent concentration.
Dot Blot Dot: Use at an assay dependent concentration.
ICC/IF ICC/IF: 1/500 - 1/3000.
IHC-P IHC-P: Use at an assay dependent concentration.
IHC-Fr IHC-Fr: Use at an assay dependent concentration.

Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (Biotin) images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (Biotin) (ab6746)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (Biotin) (ab6746)
    Ab6746 was used at dilution 1/500 with the primary antibody ab9013 in IHC-P. See the review on ab9013.

References for Rabbit polyclonal Secondary Antibody to Sheep IgG - H&L (Biotin) (ab6746)

This product has been referenced in:
  • Lindersson E  et al. p25alpha Stimulates alpha-synuclein aggregation and is co-localized with aggregated alpha-synuclein in alpha-synucleinopathies. J Biol Chem 280:5703-15 (2005). Read more (PubMed: 15590652) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"