Overview
- Product nameAnti-ABLIM3 antibodySee all ABLIM3 primary antibodies ...
- DescriptionMouse polyclonal to ABLIM3
- Tested applicationsWB, IHC-Fr more details
- Species reactivityReacts with: Mouse
Predicted to work with: Human - Immunogen
Full length protein, corresponding to amino acids 1-544 of Human ABLIM3
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferConstituent: PBS
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Concentration information loading... - PurityProtein A purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab67721 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: 1/500 - 1/1000. Predicted molecular weight: 78 kDa. |
| IHC-Fr | IHC-Fr: Use at an assay dependent concentration. |
Target
- FunctionMay act as scaffold protein. May stimulate ABRA activity and ABRA-dependent SRF transcriptional activity.
- Tissue specificityExpressed predominantly in heart and brain.
- Sequence similaritiesContains 1 HP (headpiece) domain.
Contains 4 LIM zinc-binding domains. - Cellular localizationCytoplasm.
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Database links
- Entrez Gene: 22885 Human
- Entrez Gene: 319713 Mouse
- Omim: 611305 Human
- SwissProt: O94929 Human
- SwissProt: Q69ZX8 Mouse
- Unigene: 49688 Human
- Unigene: 329478 Mouse
Target information above from: UniProt accession
O94929
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- abLIM-3 antibodyAblim3 antibodyABLM3_HUMAN antibody
- Actin binding LIM protein 3 antibodyActin binding LIM protein family member 3 antibodyActin-binding LIM protein 3 antibodyActin-binding LIM protein family member 3 antibodyHMFN1661 antibodyKIAA0843 antibody
see all
Anti-ABLIM3 antibody images
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Western blot - ABLIM3 antibody (ab67721)All lanes : Anti-ABLIM3 antibody (ab67721) at 1/500 dilution
Lane 1 : ABLIM3 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP conjugated at 1/2500 dilution
Predicted band size : 78 kDa -
Immunohistochemistry (Frozen sections) - Anti-ABLIM3 antibody (ab67721)Image courtesy of Dr Amar Sahay by Abreview.ab67721 staining ABLIM3 in murine cerebellum tissue by Immunohistochemistry (Frozen sections).Tissue was fixed with acetone, blocked with 1% BSA for 1 hour at 25°C and then incubated with ab67721 at a 1/200 dilution for 24 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated donkey anti-mouse polyclonal used at a 1/400 dilution.
References for Anti-ABLIM3 antibody (ab67721)
ab67721 has not yet been referenced specifically in any publications.
