Anti-L3MBTL3 antibody (ab68117)
- Product nameAnti-L3MBTL3 antibodySee all L3MBTL3 primary antibodies ...
- DescriptionMouse polyclonal to L3MBTL3
- Tested applicationsIP, WB, ICC/IF more details
- Species reactivityReacts with: Human
L3MBTL3 (AAH60845.1, 1 a.a. ~ 755 a.a) full-length human protein.
- Positive controlHeLa cells, transfected 293T cell lysate
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: 1X PBS, pH 7.2
- Concentration information loading...
- PurityProtein G purified
- Clonality Polyclonal
- Research Areas
Our Abpromise guarantee covers the use of ab68117 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||IP: Use at an assay dependent concentration.|
|WB||WB: 1/500 - 1/1000. Detects a band of approximately 100 kDa (predicted molecular weight: 88 kDa).|
|ICC/IF||ICC/IF: Use a concentration of 10 µg/ml.|
- RelevancePutative Polycomb group (PcG) protein. PcG proteins maintain the transcriptionally repressive state of genes, probably via a modification of chromatin, rendering it heritably changed in its expressibility. Required for normal maturation of myeloid progenitor cells.
- Cellular localizationNuclear
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Anti-L3MBTL3 antibody images
All lanes : Anti-L3MBTL3 antibody (ab68117) at 1/500 dilution
Lane 1 : L3MBTL3 transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Goat Anti-Mouse IgG (H&L)-HRP Conjugate secondary antibody at 1/2500 dilution
Predicted band size : 88 kDa
Observed band size : 100 kDa (why is the actual band size different from the predicted?)
Immunofluorescence analysis of HeLa cells using purified ab68117 at 10 µg/ml followed by fluorescein conjugated secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilised with 0.1% Triton X-100.
ab68117 used in Immunoprecipitation.Whole cell lysate from cells overexpressing L3MBTL3 tagged with GFP was loaded at 250000µg.Protein G was used in the Immunoprecipitation step.ab68117 used at 20µl/mg lysate.The western blot antibody was a rat anti-GFP polyclonal conjugated to agarose, used at a 1/5000 dilution.
References for Anti-L3MBTL3 antibody (ab68117)
ab68117 has not yet been referenced specifically in any publications.