Overview
Product namePIK3CG + PIK3R5 protein (Active)
Protein descriptionHuman recombinant Full Length proteins: PI 3 Kinase catalytic subunit gamma and PIK3R5. The PI 3 Kinase catalytic subunit gamma carries a N-terminal X6His Tag, whereas the PIK3R5 adaptor subunit carries a N-terminal enzyme tag.
The molecular weight for PI 3 Kinase catalytic subunit gamma chain is 126.3 kDa (without Tag) and the PIK3R5 chain 101 kDa (without Tag). Swiss Prot Ids: P48736 (catalytic unit) and Q8WYR1 (PIK3R5 subunit)
Source: Sf9 insect cells.
Expression hostBaculovirus infected sf9 cells
Properties
Purity> 95
% by SDS-PAGE
Biological activityActivity: ~ 3 nmol/mg/min using phosphatidylinositol as the substrate.
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Storage bufferPreservative: None
Constituents: 50% Glycerol, 25mM Sodium chloride, 25mM HEPES, 2.5mM Magnesium chloride, pH 8.0
Concentration information loading...
Additional notesFor long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab68181
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
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Application
|
Notes |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent dilution. |
Protein info
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Alternative names
PI3 kinase catalitic gamma polypetidePI3 kinase regulatory subunit 5PI3KPI3KgammaPIK3R5Protein FOAP 2
see all
RelevancePI3 Kinase catalytic subunit gamma belongs to a family of lipid kinases that are implicated in signal transduction. It is an important modulator of extracellular signals, including those elicited by E-cadherin-mediated cell-cell adhesion, which plays an important role in maintenance of the structural and functional integrity of epithelia. In addition to its role in promoting assembly of adherens junctions, the protein is thought to play a pivotal role in the regulation of cytotoxicity in NK cells. It forms a heterodimer of a catalytic subunit (PI3 Kinase catalytic subunit gamma/p120) with the regulatory (PIK3R5/p101) subunit.
PI3KR5 is a receptor-regulated class I phosphoinositide 3-kinases (PI3Ks) phosphorylate the membrane lipid phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2) to PtdIns(3,4,5)P3, which in turn recruits and activates cytosolic effectors involved in proliferation, survival, or chemotaxis. PIK3R5 is a PI3K regulatory subunit.
Cellular localizationPIK3R5: Nucleus. Cytoplasm. Membrane; Peripheral membrane protein.
References for PIK3CG + PIK3R5 protein (Active) (ab68181)
ab68181
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
