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Goat F(ab) polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin) (ab6822)

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Overview

  • Product nameGoat F(ab) polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin)See all IgG secondary antibodies ...
  • Target speciesMouse
  • SpecificityAssay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin and anti-Goat Serum. No reaction was observed against anti-Papain or anti-Goat IgG F(c).
  • Tested applicationsDot Blot, WB, ELISA, IHC, Immunomicroscopy, ICC/IF more details
  • Immunogen

    Mouse IgG whole molecule

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage bufferPreservative: 0.01% Sodium Azide
    Constituents: 10mg/ml BSA (IgG and protease free), 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Mouse IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities, papain digestion and chromatographic separation.
  • Conjugation notesBiotinamidocaproate N-Hydroxysuccinimide Ester (BAC) Biotin/Protein Ratio: 10-20 BAC molecules per Goat IgG Fab molecule
  • Clonality Polyclonal
  • IsotypeIgG
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab6822 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
Dot Blot Dot: Use at an assay dependent dilution.
WB WB: 1/2000 - 1/10000.
ELISA ELISA: 1/200000.
IHC IHC: Use at an assay dependent dilution.
Immunomicroscopy IM: Use at an assay dependent dilution.
ICC/IF ICC/IF: 1/1000 - 1/5000.

References for Goat F(ab) polyclonal Secondary Antibody to Mouse IgG - H&L (Biotin) (ab6822)

ab6822 has not yet been referenced specifically in any publications.

Publishing research using ab6822 ? Please let us know so that we can cite the reference in this datasheet

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Submit a Question for ab6822

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Votes: Highest First

Could you please advise if there is any chance of this secondary cross reacting with rat?

Thanks for your help! J

Kind regards,

Thank you for contacting us.

This antibody should not be cross reacting with Rat. If customer is thinking about a substitution of anti rat with this antibody then please advice them, it will be a total waste of time and reagents.

I hope ...

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dear colleagues
hallo
can i ask about the optimal dilution of these products.
1-ab33923
2-ab6668
3-ab6822
4-ab7481
5-ab97049

Thank you for contacting Abcam.

As with most antibodies the dilution that works best for you depends on multiple varaibles and so you should do some titrations to find the best for you, but I placed a good starting point next to each.

...

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"