Overview
Product nameHepatitis A virus VP3 protein
Protein descriptionRecombinant fragment containing the Hepatitis A Virus VP3 immunodominant regions, amino acids 304-415.
Expression hostE. coli
Properties
Purity> 90
% by SDS-PAGE
Purification notesThis protein was purified by proprietary chromatographic techniques.
Biological activityThis protein is immunoreactive with sera from HAV-infected individuals.
FormLiquid
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.This product is an active protein and may elicit a biological response in vivo, handle with caution.
Storage bufferPreservative: None
Constituents: 50% Glycerol, 0.1% SDS, 10mM CBB, pH 9.6.
Concentration information loading...
Research Areas
Applications
Our Abpromise guarantee covers the use of
ab68371
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|
Application
|
Notes |
| WB |
WB: Use at an assay dependent dilution. Predicted molecular weight: 18 kDa. |
| ELISA |
ELISA: Use at an assay dependent dilution. |
| SDS-PAGE |
SDS-PAGE: Use at an assay dependent dilution. |
Protein info
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Alternative names
HAV VP3Hepatitis A VP3 proteinVirion protein 3VP3
RelevanceHepatitis A Virus (HAV) is a 27nm nonenveloped, spherical, positive stranded RNA virus, classified within the genus hepatovirus of the picornavirus family and is among the smallest and structurally simplest of the RNA animal viruses. A single large polyprotein is expressed from a large open reading frame extending through most of the genomic RNA. This polyprotein is subsequently cleaved by a viral protease (3Cpro) to form three (possibly four) capsid proteins and several nonstructural proteins. HAV genomic replication occurs exclusively in the cytoplasm of the infected hepatocyte by a mechanism involving an RNA-dependent RNA polymerase.
Though he clinical picture of viral (A) hepatitis is extremely variable - ranging from asymptotic infection without jaundice to a fulminating disease and death in a few days, Hepatitis A is usually a mild illness and does not lead to chronic or persistent hepatitis. HAV is transmitted by the orofecal route and is still endemic throughout much of the world, including the western world. HAV exists worldwide as a single serotype with a small degree of antigenetic variation. Present evidence suggests that immunity acquired naturally (or from an inactivated hepatitis A vaccine) will protect against all human HAV strains.
Cellular localizationCytoplasmic
References for Hepatitis A virus VP3 protein (ab68371)
ab68371
has not yet been referenced specifically in any publications.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
