Anti-non-muscle Myosin IIB antibody [3H2] (ab684)

Overview

• Product nameAnti-non-muscle Myosin IIB antibody [3H2]See all non-muscle Myosin IIB primary antibodies ...
• Description
  Mouse monoclonal [3H2] to non-muscle Myosin IIB
• SpecificityThis antibody reacts with non-muscle myosin heavy chain (SMemb).
• Tested applicationsIHC-P, ICC/IF, WB, IHC-Fr, Flow Cyt more details
• Species reactivity
  Reacts with: Mouse, Rat, Rabbit, Hamster, Human
  
• Immunogen

  Synthetic peptide: TSDVNETQPPQSE, corresponding to C (beta) terminal amino acids 1964-1976 of Human non-muscle Myosin IIB.

  Run BLAST with Run BLAST with

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferAscitic fluid
• Concentration information loading...
• PurityAscites
• Clonality Monoclonal
• Clone number3H2
• IsotypeIgG2b
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Motor Proteins
  • Myosin

Applications

Target

• FunctionCellular myosin that appears to play a role in cytokinesis, cell shape, and specialized functions such as secretion and capping. Involved with LARP6 in the stabilization of type I collagen mRNAs for CO1A1 and CO1A2.
• Tissue specificityIsoform 1 is expressed in cerebellum and spinal chord. Isoform 2 is expressed in cerebrum and retina. Isoform 3 is expressed in the cerebrum and to a much lower extent in cerebellum.
• Sequence similaritiesContains 1 IQ domain.
  Contains 1 myosin head-like domain.
• DomainThe rodlike tail sequence is highly repetitive, showing cycles of a 28-residue repeat pattern composed of 4 heptapeptides, characteristic for alpha-helical coiled coils.
• Post-translational
  modificationsPhosphorylated by ABL2.

Target information above from: UniProt accession P35580 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 4628 Human
  • Entrez Gene: 77579 Mouse
  • Entrez Gene: 79433 Rat
  • Omim: 160776 Human
  • SwissProt: P35580 Human
  • SwissProt: Q5SV64 Mouse
  • SwissProt: Q8BXF2 Mouse
  • SwissProt: Q61879 Mouse

  • SwissProt: Q9JLT0 Rat
  • Unigene: 16355 Human
  • Unigene: 218233 Mouse
  • Unigene: 98166 Rat

  see all

• Alternative names
  Cellular myosin heavy chain antibodyCellular myosin heavy chain type B antibodyCellular myosin heavy chain type B type B antibody

  MGC134913 antibodyMGC134914 antibodyMYH 10 antibodyMyh10 antibodyMYH10_HUMAN antibodyMyosin 10 antibodyMyosin heavy chain 10 antibodyMyosin heavy chain 10 non muscle antibodyMyosin heavy chain antibodyMyosin heavy chain non muscle 11b antibodyMyosin heavy chain nonmuscle IIb antibodyMyosin heavy chain nonmuscle type B antibodyMyosin heavy polypeptide 10 non muscle antibodyMyosin-10 antibodyMyosin10 antibodyNMMHC B antibodyNMMHC II b antibodyNMMHC II-b antibodyNMMHC IIB antibodyNMMHC-B antibodyNMMHC-IIB antibodyNMMHCB antibodyNon muscle myosin heavy chain B antibodyNon muscle myosin heavy chain IIB antibodyNon muscle myosin II heavy chain B antibodynon-muscle IIb antibodyNon-muscle myosin heavy chain B antibodyNon-muscle myosin heavy chain IIb antibodyNonmuscle myosin heavy chain B antibodyNonmuscle myosin heavy chain IIB antibodyNonmuscle myosin II heavy chain B antibodytype B antibody

  see all

Anti-non-muscle Myosin IIB antibody [3H2] images

• 

  Immunocytochemistry/ Immunofluorescence - Anti-non-muscle Myosin IIB antibody [3H2] (ab684)
  ICC/IF image of ab684 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1% BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab684, 1 µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in Hek293, HepG2 and MCF7 cells.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-non-muscle Myosin IIB antibody [3H2] (ab684)
  Ab684 staining nnon-muscle Myosin IIB in Human colon.
  Left panel: with primary antibody at 4 ug/ml. Right panel: isotype control.
  Sections were stained using an automated system (DAKO Autostainer Plus), at room temperature: sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffers citrate pH6.1. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako envision flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be required.
• 

  Flow Cytometry - Anti-non-muscle Myosin IIB antibody [3H2] (ab684)
  Overlay histogram showing HeLa cells stained with ab684 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Triton for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab684, 1µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x10⁶ cells ) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Triton used under the same conditions.