Anti-Bcl2 antibody [100/D5] (ab692)

Overview

• Product nameAnti-Bcl2 antibody [100/D5]See all Bcl2 primary antibodies ...
• Description
  Mouse monoclonal [100/D5] to Bcl2
• SpecificityThis antibody recognises a protein of 25-26kDa, identified as the bcl-2a oncoprotein. It does not cross-react with bcl-x or bax protein.
• Tested applicationsFlow Cyt, IHC-P, IHC-Fr, IP, WB, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Human
  

  Does not react with

  Rat
• Immunogen

  A Synthetic peptide, aa 41-54 (GAAPAPGIFSSQPG-Cys), of human bcl-2 protein.

  Run BLAST with Run BLAST with
• Positive controlFollicular lymphomas or tonsil.

Properties

• FormLiquid
• Storage instructionsStore at +4°C.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 1% BSA, Tissue culture supernatant, PBS, pH 7.3
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Monoclonal
• Clone number100/D5
• MyelomaP3-NS1/1-Ag4-1
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Hypoxia

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Apoptosis

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Bcl 2 family

  • Cancer
  • Tumor biomarkers
  • Oncoproteins

  • Cancer
  • Cancer Metabolism
  • Response to hypoxia

  • Cancer
  • Cancer Metabolism
  • Cellular metabolic process

  • Cancer
  • Oncoproteins/suppressors
  • Oncoproteins
  • Cell survival & death

  • Signal Transduction
  • Metabolism
  • Mitochondrial

  • Cell Biology
  • Apoptosis
  • Intracellular
  • Bcl2 Family

Applications

Target

• FunctionSuppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).
• Tissue specificityExpressed in a variety of tissues.
• Involvement in diseaseNote=A chromosomal aberration involving BCL2 has been found in chronic lymphatic leukemia. Translocation t(14;18)(q32;q21) with immunoglobulin gene regions. BCL2 mutations found in non-Hodgkin lymphomas carrying the chromosomal translocation could be attributed to the Ig somatic hypermutation mechanism resulting in nucleotide transitions.
• Sequence similaritiesBelongs to the Bcl-2 family.
• DomainThe BH4 motif is required for anti-apoptotic activity and for interaction with RAF1 and EGLN3.
• Post-translational
  modificationsPhosphorylation/dephosphorylation on Ser-70 regulates anti-apoptotic activity. Growth factor-stimulated phosphorylation on Ser-70 by PKC is required for the anti-apoptosis activity and occurs during the G2/M phase of the cell cycle. In the absence of growth factors, BCL2 appears to be phosphorylated by other protein kinases such as ERKs and stress-activated kinases. Phosphorylated by MAPK8/JNK1 at Thr-69, Ser-70 and Ser-87, wich stimulates starvation-induced autophagy. Dephosphorylated by protein phosphatase 2A (PP2A).
  Proteolytically cleaved by caspases during apoptosis. The cleaved protein, lacking the BH4 motif, has pro-apoptotic activity, causes the release of cytochrome c into the cytosol promoting further caspase activity.
  Monoubiquitinated by PARK2, leading to increase its stability.
• Cellular localizationMitochondrion outer membrane. Nucleus membrane. Endoplasmic reticulum membrane.

Target information above from: UniProt accession P10415 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 596 Human
  • Entrez Gene: 12043 Mouse
  • Entrez Gene: 12043 Mouse
  • Omim: 151430 Human
  • SwissProt: P10415 Human
  • SwissProt: P10417 Mouse
  • Unigene: 150749 Human
  • Unigene: 257460 Mouse

• Alternative names
  Apoptosis regulator Bcl 2 antibodyApoptosis regulator Bcl-2 antibodyApoptosis regulator Bcl2 antibody

  AW986256 antibodyB cell CLL/lymphoma 2 antibodyB cell leukemia/lymphoma 2 antibodyB cell lymphoma 2 antibodyBcl 2 antibodyBcl-2 antibodyBcl2 antibodyBCL2 protein antibodyBCL2_HUMAN antibodyC430015F12Rik antibodyD630044D05Rik antibodyD830018M01Rik antibodyLeukemia/lymphoma, B-cell, 2 antibodyOncogene B-cell leukemia 2 antibodyPPP1R50 antibodyProtein phosphatase 1, regulatory subunit 50 antibody

  see all

Anti-Bcl2 antibody [100/D5] images

• 

  Immunocytochemistry/ Immunofluorescence - Anti-Bcl2 antibody [100/D5] (ab692)
  ab692 staining Bcl2 in SK-N-SH cells treated with (R)-(-)-Deprenyl hydrochloride (Selegiline hydrochloride) (ab120604), by ICC/IF. Increase of Bcl2 expression correlates with increased concentration of (R)-(-)-Deprenyl hydrochloride (Selegiline hydrochloride), as described in literature.
  The cells were incubated at 37°C for 3h in media containing different concentrations of ab120604 ((R)-(-)-Deprenyl hydrochloride (Selegiline hydrochloride)) in DMSO, fixed with 4% formaldehyde for 10 minutes at room temperature and blocked with PBS containing 10% goat serum, 0.3 M glycine, 1% BSA and 0.1% tween for 2h at room temperature. Staining of the treated cells with ab692 (5 μg/ml) was performed overnight at 4°C in PBS containing 1% BSA and 0.1% tween. A DyLight 488 anti-mouse polyclonal antibody (ab96879) at 1/250 dilution was used as the secondary antibody. Nuclei were counterstained with DAPI and are shown in blue.
• 

  Immunohistochemistry (Frozen sections) - Bcl2 antibody [100/D5] (ab692)

  Bcl2 was localized distinctly in specific cytoplasmic regions in human epithelium (especially in the lower and outmost layers). This image was kindly supplied as part of the review submitted by Marko Nykanen.

   

   

• 

  Immunoprecipitation - Bcl2 antibody [100/D5] (ab692)This image is courtesy of an anonymous Abreview

  ab692 diluted 1/100 in BSA was incubated with whole cell lysate of human lymphocytes and a Protein G matrix for 12 hours at 4°C to achieve immunoprecipitation.  1,000,000 cells were present in the lysate.

  A modified RIPA buffer was used to lyse cells.

  The IP was washed with PBS/Triton.

  ab692 was used at a 1/1000 dilution for the Western Blot step.

  See Abreview

• 

  Flow Cytometry-Bcl2 antibody [100/D5](ab692)
  Overlay histogram showing Jurkat cells stained with ab692 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab692, 1/10 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Jurkat cells fixed with methanol (5 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.