Overview
- Product nameAnti-Klotho antibodySee all Klotho primary antibodies ...
- DescriptionRabbit polyclonal to Klotho
- Tested applicationsWB, IHC-P more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Non Human Primates - Immunogen
Synthetic peptide conjugated to KLH derived from within residues 800 - 900 of Human Klotho.
(Peptide available as ab75021.)
- Positive controlThis antibody gave a positive signal in the following whole cell lysates: HepG2, MOLT4
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab69208 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | |
| IHC-P |
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionMay have weak glycosidase activity towards glucuronylated steroids. However, it lacks essential active site Glu residues at positions 239 and 872, suggesting it may be inactive as a glycosidase in vivo. May be involved in the regulation of calcium and phosphorus homeostasis by inhibiting the synthesis of active vitamin D (By similarity). Essential factor for the specific interaction between FGF23 and FGFR1.
The Klotho peptide generated by cleavage of the membrane-bound isoform may be an anti-aging circulating hormone which would extend life span by inhibiting insulin/IGF1 signaling. - Tissue specificityPresent in cortical renal tubules (at protein level). Soluble peptide is present in serum and cerebrospinal fluid. Expressed in kidney, placenta, small intestine and prostate. Down-regulated in renal cell carcinomas, hepatocellular carcinomas, and in chronic renal failure kidney.
- Involvement in diseaseDefects in KL are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC) [MIM:211900]. A severe metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Some patients manifest recurrent, transient, painful swellings of the long bones associated with the radiographic findings of periosteal reaction and cortical hyperostosis and absence of skin involvement.
- Sequence similaritiesBelongs to the glycosyl hydrolase 1 family. Klotho subfamily.
- DomainContains 2 glycosyl hydrolase 1 regions. However, the first region lacks the essential Glu active site residue at position 239, and the second one lacks the essential Glu active site residue at position 872.
- Post-translational
modificationsN-glycosylated. - Cellular localizationSecreted and Cell membrane. Isoform 1 shedding leads to a soluble peptide.
-
Database links
- Entrez Gene: 9365 Human
- Entrez Gene: 16591 Mouse
- Entrez Gene: 83504 Rat
- Omim: 604824 Human
- SwissProt: Q9UEF7 Human
- SwissProt: O35082 Mouse
- SwissProt: Q9Z2Y9 Rat
- Unigene: 524953 Human
- Unigene: 6500 Mouse
- Unigene: 30061 Rat
see all
Target information above from: UniProt accession
Q9UEF7
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
-
Alternative names
- KL antibodyKLOT_HUMAN antibodyKlotho peptide antibody
Anti-Klotho antibody images
-
Western blot - Klotho antibody (ab69208)All lanes : Anti-Klotho antibody (ab69208) at 1 µg/ml
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : MOLT4 (Human acute lymphoblastic leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 116 kDa
Observed band size : 116 kDa
Additional bands at : 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time : 12 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Klotho antibody (ab69208)This image is a courtesy of Abreview submitted by Antibody Solutions Ltdab69208 staining Klotho in human kidney tissue sections by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Tissue underwent fixation in formaldehyde, heat mediated antigen retrieval and blocking (5 minutes/peroxidase block and 10 minutes/protein block) for 15 minutes at 200C was performed. The primary antibody was diluted 1/250 and incubated with the sample for 45 minutes at 200C. A HRP conjugated goat polyclonal to rabbit IgG was used undiluted as secondary antibody.
References for Anti-Klotho antibody (ab69208)
ab69208 has not yet been referenced specifically in any publications.
