Anti-NDUFS2 antibody (ab70408)

Overview

• Product nameAnti-NDUFS2 antibodySee all NDUFS2 primary antibodies ...
• Description
  Mouse polyclonal to NDUFS2
• Tested applicationsWB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Mouse, Goat, Cow
• Immunogen

  Full length human NDUFS2 protein (NP_004541.1)

• Positive controlNDUFS2 transfected 293T cell lysate, human kidney lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 1X PBS, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Oxidative stress

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Integration of energy

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Oxidative phosphorylation
  • Complex I

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Integration of energy metabolism

  • Signal Transduction
  • Metabolism
  • Mitochondrial

  • Tags & Cell Markers
  • Subcellular Markers
  • Organelles
  • Mitochondria

  • Cell Biology
  • Other Antibodies
  • Oxidative Stress

Applications

Target

• FunctionCore subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
• Involvement in diseaseDefects in NDUFS2 are a cause of mitochondrial complex I deficiency (MT-C1D) [MIM:252010]. A disorder of the mitochondrial respiratory chain that causes a wide range of clinical disorders, from lethal neonatal disease to adult-onset neurodegenerative disorders. Phenotypes include macrocephaly with progressive leukodystrophy, non-specific encephalopathy, cardiomyopathy, myopathy, liver disease, Leigh syndrome, Leber hereditary optic neuropathy, and some forms of Parkinson disease.
• Sequence similaritiesBelongs to the complex I 49 kDa subunit family.
• Cellular localizationMitochondrion inner membrane.

Target information above from: UniProt accession O75306 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 327697 Cow
  • Entrez Gene: 4720 Human
  • Entrez Gene: 226646 Mouse
  • Omim: 602985 Human
  • SwissProt: O75306 Human
  • SwissProt: Q91WD5 Mouse
  • Unigene: 173611 Human
  • Unigene: 21669 Mouse

• Alternative names
  CI 49kD antibodyCI-49kD antibodyComplex I 49kD antibody

  Complex I-49kD antibodymitochondrial antibodyNADH dehydrogenase [ubiquinone] iron sulfur protein 2, mitochondrial antibodyNADH dehydrogenase [ubiquinone] iron-sulfur protein 2 antibodyNADH ubiquinone oxidoreductase 49 kDa subunit antibodyNADH-ubiquinone oxidoreductase 49 kDa subunit antibodyNdufs2 antibodyNDUS2_HUMAN antibody

  see all

Anti-NDUFS2 antibody images

• 

  Western blot - NDUFS2 antibody (ab70408)
  Anti-NDUFS2 antibody (ab70408) at 1/500 dilution + human kidney lysate at 25 µg
  
  Secondary
  Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
  
  Predicted band size : 53 kDa
  Observed band size : 48 kDa (why is the actual band size different from the predicted?)
  
• 

  Western blot - NDUFS2 antibody (ab70408)
  All lanes : Anti-NDUFS2 antibody (ab70408) at 1/500 dilution
  
  Lane 1 : NDUFS2 transfected 293T cell lysate
  Lane 2 : non transfected 293T cell lysate
  
  Lysates/proteins at 25 µg per lane.
  
  Secondary
  Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
  
  Predicted band size : 53 kDa
  Observed band size : 48 kDa (why is the actual band size different from the predicted?)
  
• 

  Western blot - NDUFS2 antibody (ab70408)
  All lanes : Anti-NDUFS2 antibody (ab70408) at 1/1000 dilution
  
  Lane 1 : Mouse endometrial whole cell lysate at 20 µg
  Lane 2 : Mouse 3T3L-1 whole cell lysate at 20 µg
  Lane 3 : Goat MG whole cell lysate at 30 µg
  Lane 4 : Bovine MAC-T whole cell lysate at 30 µg
  
  Secondary
  HRP-conjugated Goat polyclonal to mouse IgG at 1/1000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 53 kDa
  Observed band size : 48 kDa (why is the actual band size different from the predicted?)
  
  
  Exposure time : 2 minutes

  This image is a courtesy of YongCheng Jin

  See Abreview