Overview
- Product nameAnti-PECI antibodySee all PECI primary antibodies ...
- DescriptionMouse polyclonal to PECI
- Tested applicationsICC/IF, WB more details
- Species reactivityReacts with: Human
- Immunogen
Full length protein, corresponding to amino acids 1-364 of human PECI
- Positive controlHuman liver mitochondrial or peroxisome lysate and PECI transfected 293T mitochondrial or peroxisome lysate
Properties
- FormLiquid
- Storage instructionsStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
- Storage bufferPreservative: None
Constituents: 1X PBS, pH 7.2 -
Concentration information loading... - PurityProtein G purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab70512 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ICC/IF | ICC/IF: Use a concentration of 10 µg/ml. |
| WB | WB: 1/500 - 1/1000. Detects a band of approximately 40 kDa (predicted molecular weight: 40 kDa). |
Target
- FunctionAble to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species. Has a preference for 3-trans substrates.
- Tissue specificityAbundant in heart, skeletal muscle and liver. Expressed in CD34(+) T-cells and CD34(+) bone marrow cells.
- Sequence similaritiesIn the C-terminal section; belongs to the enoyl-CoA hydratase/isomerase family.
Contains 1 ACB (acyl-CoA-binding) domain. - Cellular localizationMitochondrion and Peroxisome matrix.
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Database links
- Entrez Gene: 10455 Human
- Omim: 608024 Human
- SwissProt: O75521 Human
- Unigene: 15250 Human
Target information above from: UniProt accession
O75521
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- 2-trans-enoyl-CoA isomerase antibodyACBD2 antibodyacyl Coenzyme A binding domain containing 2 antibody
- D2-enoyl-CoA isomerase antibodyD3 antibodyD3 D2 enoyl CoA isomerase antibodyDBI related protein 1 antibodyDBI-related protein 1 antibodydelta(2)-enoyl-CoA isomerase antibodyDelta(3) antibodyDelta(3) delta(2) enoyl CoA isomerase antibodyDiazepam-binding inhibitor-related protein 1 antibodyDodecenoyl CoA isomerase antibodyDodecenoyl-CoA isomerase antibodyDRS 1 antibodyDRS-1 antibodyDRS1 antibodyEci2 antibodyECI2_HUMAN antibodyEnoyl-CoA delta isomerase 2 antibodyHCA88 antibodyHepatocellular carcinoma associated antigen 88 antibodyHepatocellular carcinoma-associated antigen 88 antibodymitochondrial antibodypECI antibodyPeroxisomal 3 antibodyPeroxisomal 3,2-trans-enoyl-CoA isomerase antibodyPeroxisomal D3 D2 enoyl CoA isomerase antibodyRenal carcinoma antigen NY REN 1 antibodyRenal carcinoma antigen NY-REN-1 antibody
see all
Anti-PECI antibody images
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Western blot - PECI antibody (ab70512)Anti-PECI antibody (ab70512) at 1/500 dilution + human liver tissue lysate at 25 µg
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 40 kDa
Observed band size : 40 kDa -
Western blot - PECI antibody (ab70512)All lanes : Anti-PECI antibody (ab70512) at 1/500 dilution
Lane 1 : PECI transfected 293T cell lysate
Lane 2 : Non transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
Secondary
Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
Predicted band size : 40 kDa
Observed band size : 40 kDa -
Immunocytochemistry/ Immunofluorescence - PECI antibody (ab70512)ab70512 at 10µg/ml staining PECl in Hela cells by Immunocytochemistry/Immunofluorescence.
References for Anti-PECI antibody (ab70512)
ab70512 has not yet been referenced specifically in any publications.
