Anti-LATS1/WARTS antibody (ab70562)
Key features and details
- Rabbit polyclonal to LATS1/WARTS
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-LATS1/WARTS antibody
See all LATS1/WARTS primary antibodies -
Description
Rabbit polyclonal to LATS1/WARTS -
Host species
Rabbit -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Rat, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
Immunogen
Synthetic peptide corresponding to Human LATS1/WARTS aa 1075-1130 (C terminal).
Database link: O95835 -
Positive control
- WB: HeLa whole cell lysate, mouse TCMK-1, 4T1 and CT26.WT whole cell lysate
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7
Preservative: 0.09% Sodium azide
Constituents: 1.815% Tris, 1.764% Sodium citrate, 0.021% PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab70562 was affinity purified using an epitope specific to LATS1/WARTS immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab70562 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
1/1000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 127 kDa).
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Notes |
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WB
1/1000 - 1/10000. Detects a band of approximately 160 kDa (predicted molecular weight: 127 kDa). |
Target
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Function
Negative regulator of YAP1 in the Hippo signaling pathway that plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Acts as a tumor suppressor which plays a critical role in maintenance of ploidy through its actions in both mitotic progression and the G1 tetraploidy checkpoint. Negatively regulates G2/M transition by down-regulating CDK1 kinase activity. Involved in the control of p53 expression. Affects cytokinesis by regulating actin polymerization through negative modulation of LIMK1. May also play a role in endocrine function. -
Tissue specificity
Expressed in all adult tissues examined except for lung and kidney. -
Sequence similarities
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain.
Contains 1 UBA domain. -
Post-translational
modificationsAutophosphorylated and phosphorylated during M-phase of the cell cycle. Phosphorylated by STK3/MST2 at Ser-909 and Thr-1079, which results in its activation. Phosphorylation at Ser-464 by NUAK1 and NUAK2 leads to decreased protein level and is required to regulate cellular senescence and cellular ploidy. -
Cellular localization
Cytoplasm, cytoskeleton, microtubule organizing center, centrosome. Localizes to the centrosomes throughout interphase but migrates to the mitotic apparatus, including spindle pole bodies, mitotic spindle, and midbody, during mitosis. - Information by UniProt
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Database links
- Entrez Gene: 9113 Human
- Entrez Gene: 16798 Mouse
- Entrez Gene: 308265 Rat
- Omim: 603473 Human
- SwissProt: O95835 Human
- SwissProt: Q8BYR2 Mouse
- Unigene: 549084 Human
- Unigene: 34083 Mouse
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Alternative names
- h-warts antibody
- Large tumor suppressor homolog 1 antibody
- LATS large tumor suppressor homolog 1 antibody
see all
Images
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All lanes : Anti-LATS1/WARTS antibody (ab70562) at 1 µg/ml
Lane 1 : TCMK-1 whole cell lysate
Lane 2 : 4T1 whole cell lysate
Lane 3 : CT26.WT whole cell lysate
Lysates/proteins at 50 µg per lane.
Predicted band size: 127 kDa
Exposure time: 30 seconds -
All lanes : Anti-LATS1/WARTS antibody (ab70562) at 0.1 µg/ml
Lane 1 : HeLa at 50 µg
Lane 2 : HeLa at 15 µg
Lane 3 : HeLa at 5 µg
Predicted band size: 127 kDa
Exposure time: 30 seconds
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab70562 has been referenced in 10 publications.
- Jin X et al. MST1 inhibits the progression of breast cancer by regulating the Hippo signaling pathway and may serve as a prognostic biomarker. Mol Med Rep 23:N/A (2021). PubMed: 33760220
- Luo J et al. PKM2 regulates proliferation and apoptosis through the Hippo pathway in oral tongue squamous cell carcinoma. Oncol Lett 21:461 (2021). PubMed: 33907571
- Liu G et al. Hsa_circ_0085576 promotes clear cell renal cell carcinoma tumorigenesis and metastasis through the miR-498/YAP1 axis. Aging (Albany NY) 12:11530-11549 (2020). PubMed: 32541093
- Popow O et al. Identification of Endogenous Adenomatous Polyposis Coli Interaction Partners and ß-Catenin-Independent Targets by Proteomics. Mol Cancer Res 17:1828-1841 (2019). PubMed: 31160382
- Zhang C et al. Dysregulation of YAP by the Hippo pathway is involved in intervertebral disc degeneration, cell contact inhibition, and cell senescence. Oncotarget 9:2175-2192 (2018). PubMed: 29416763
- Lin M et al. Yes-associated protein mediates angiotensin II-induced vascular smooth muscle cell phenotypic modulation and hypertensive vascular remodelling. Cell Prolif 51:e12517 (2018). PubMed: 30156340
- Zhang C et al. AMOT130 linking F-actin to YAP is involved in intervertebral disc degeneration. Cell Prolif N/A:e12492 (2018). PubMed: 30039887
- Sorrentino G et al. Glucocorticoid receptor signalling activates YAP in breast cancer. Nat Commun 8:14073 (2017). PubMed: 28102225
- Vahid S et al. Molecular chaperone Hsp27 regulates the Hippo tumor suppressor pathway in cancer. Sci Rep 6:31842 (2016). PubMed: 27555231
- Liu S et al. miR-21 modulates resistance of HR-HPV positive cervical cancer cells to radiation through targeting LATS1. Biochem Biophys Res Commun 459:679-85 (2015). WB ; Human . PubMed: 25769949