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Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP), pre-adsorbed (ab7097)

Submit an Abreview Q&A (2)Specific References (1)

Overview

  • Product nameGoat polyclonal Secondary Antibody to Rat IgG - H&L (HRP), pre-adsorbedSee all IgG secondary antibodies ...
  • Target speciesRat
  • Tested applicationsICC/IF, IHC-P, IHC-Fr, WB, ELISA, Dot Blot more details
  • Minimal
    cross-reactivity
    Chicken, Cow, Goat, Guinea pig, Hamster, Horse, Human, Mouse, Rabbit, Sheep more details
  • Immunogen

    Full length native Rat IgG (purified).

Properties

  • FormLiquid
  • Storage instructionsStore at +4°C in the dark. Do not freeze.
  • Storage bufferPreservative: 0.01% Gentamicin Sulfate
    Constituents: 10mg/ml BSA, 0.15M Sodium Chloride, 0.02M Potassium Phosphate. pH 7.2
  • Concentration information loading...
  • PurityIgG fraction
  • Purification notesThis product was prepared from monospecific antiserum by immunoaffinity chromatography using Rat IgG coupled to agarose beads followed by solid phase adsorption(s) to remove any unwanted reactivities.
  • Conjugation notesHorseradish Peroxidase (HRP)
  • Clonality Polyclonal
  • Research Areas

Applications

Our Abpromise guarantee covers the use of ab7097 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
ICC/IF ICC/IF: 1/500 - 1/1500.
IHC-P IHC-P: Use at an assay dependent dilution.
IHC-Fr IHC-Fr: Use at an assay dependent dilution.
WB WB: Use at an assay dependent dilution.
ELISA ELISA: 1/50000.
Dot Blot Dot: Use at an assay dependent dilution.

Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP), pre-adsorbed images

  • - Rat IgG antibody - H&L - Pre-Adsorbed (ab7097)
    - Rat IgG antibody - H&L - Pre-Adsorbed (ab7097)

    Whole mount immunostaining of E11.5 mouse embryo  (Blood vessels in the embryonic head). This staining was performed with rat monoclonal antibody to Pecam followed by staining with ab 7097 (HRP conjugated).

    This picture was kindly supplied as part of the review submitted by Helen Makarenkova.

References for Goat polyclonal Secondary Antibody to Rat IgG - H&L (HRP), pre-adsorbed (ab7097)

This product has been referenced in:
  • Weiser S  et al. Interferon-gamma synergises with tumour necrosis factor and lymphotoxin-alpha to enhance the mRNA and protein expression of adhesion molecules in mouse brain endothelial cells. Cytokine 37:84-91 (2007). Read more (PubMed: 17412608) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"