Overview
- Product nameAnti-C9 antibodySee all C9 primary antibodies ...
- DescriptionRabbit polyclonal to C9
- SpecificityThis product may also detect C9 within the C5b-9 complex, but we have no evidence to show this. Any feedback from our customers is welcome. We recommend ab55811 for specifically detecting the C5b-9 complex.
- Tested applicationsWB, IHC-P more details
- Species reactivityReacts with: Human
- Immunogen
Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human C9.
(Peptide available as ab71329.)
- Positive controlThis antibody gave a positive signal in Human Placenta Tissue Lysate
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4 -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab71330 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | WB: Use a concentration of 1 µg/ml. Detects a band of approximately 75 kDa (predicted molecular weight: 63 kDa).Can be blocked with C9 peptide (ab71329). |
| IHC-P | IHC-P: Use at an assay dependent concentration. |
Target
- FunctionConstituent of the membrane attack complex (MAC) that plays a key role in the innate and adaptive immune response by forming pores in the plasma membrane of target cells. C9 is the pore-forming subunit of the MAC.
- Tissue specificityPlasma.
- Involvement in diseaseDefects in C9 are a cause of complement component 9 deficiency (C9D) [MIM:613825]. A rare defect of the complement classical pathway associated with susceptibility to severe recurrent infections, predominantly by Neisseria gonorrhoeae or Neisseria meningitidis.
- Sequence similaritiesBelongs to the complement C6/C7/C8/C9 family.
Contains 1 EGF-like domain.
Contains 1 LDL-receptor class A domain.
Contains 1 MACPF domain.
Contains 1 TSP type-1 domain. - Post-translational
modificationsThrombin cleaves factor C9 to produce C9a and C9b.
Phosphorylation sites are present in the extracelllular medium. - Cellular localizationSecreted. Cell membrane. Secreted as soluble monomer. Oligomerizes at target membranes, forming a pre-pore. A conformation change then leads to the formation of a 100 Angstrom diameter pore.
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Database links
- Entrez Gene: 735 Human
- Omim: 120940 Human
- SwissProt: P02748 Human
- Unigene: 654443 Human
Target information above from: UniProt accession
P02748
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
.
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Alternative names
- C9 antibodyC9 deficiency antibodyC9 deficiency with dermatomyositis antibody
- CO9_HUMAN antibodyComplement component 9 antibodyComplement component 9 deficiency antibodyComplement component C9 antibodyComplement component C9b antibodyMAC antibodyMembrane attack complex antibodyTCC antibodyTerminal complement complex antibody
see all
Anti-C9 antibody images
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Western blot - Anti-C9 antibody (ab71330)Anti-C9 antibody (ab71330) at 1 µg/ml +Placenta (Human) Tissue Lysate - adult normal tissue (ab29745) at 10 µg
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Performed under reducing conditions.
Predicted band size : 63 kDa
Observed band size : 75 kDa (why is the actual band size different from the predicted?)
Exposure time : 20 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C9 antibody (ab71330)ab71330, at a 1/2000 dilution, staining human C9 in placenta tissue by Immunohistochemistry, Formalin fixed Paraffin embedded tissue.
References for Anti-C9 antibody (ab71330)
ab71330 has not yet been referenced specifically in any publications.
