Anti-DNAJC3 antibody (ab72885)

Overview

• Product nameAnti-DNAJC3 antibodySee all DNAJC3 primary antibodies ...
• Description
  Rabbit polyclonal to DNAJC3
• Tested applicationsICC/IF, WB more details
• Species reactivity
  Reacts with: Rat, Human
  Predicted to work with: Mouse
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 250 - 350 of Human DNAJC3.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab72884.)

• Positive controlThis antibody gives a positive signal in the following lysates: Rat Thymus Tissue TE 671 Whole Cell Y79 Whole Cell Hela - Staurosporine Treated (24hr, 500nM) Jurkat - Staurosporine Treated (24hr, 500nM).

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Protein Trafficking
  • Chaperones
  • Other Chaperones

  • Microbiology
  • Interspecies Interaction
  • Host Virus Interaction

  • Epigenetics and Nuclear Signaling
  • DNA / RNA
  • Translation
  • Regulation

Applications

Target

• FunctionInvolved in the unfolded protein response (UPR) during ER stress. Co-chaperone of HSPA8/HSC70, it stimulates its ATPase activity. May inhibit both the autophosphorylation of EIF2AK2/PKR and the ability of EIF2AK2 to catalyze phosphorylation of the EIF2A. May inhibit EIF2AK3/PERK activity.
• Tissue specificityWidely expressed with high level in the pancreas and testis. Also expressed in cell lines with different levels.
• Sequence similaritiesContains 1 J domain.
  Contains 9 TPR repeats.
• DomainThe J domain mediates interaction with HSPA8.
• Cellular localizationEndoplasmic reticulum.

Target information above from: UniProt accession Q13217 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5611 Human
  • Entrez Gene: 100037258 Mouse
  • Entrez Gene: 63880 Rat
  • Omim: 601184 Human
  • SwissProt: Q13217 Human
  • SwissProt: Q91YW3 Mouse
  • SwissProt: Q9R0T3 Rat
  • Unigene: 59214 Human

  • Unigene: 12616 Mouse
  • Unigene: 162234 Rat

  see all

• Alternative names
  DnaJ (Hsp40) homolog subfamily C member 3 antibodyDnaJ homolog subfamily C member 3 antibodyDNAJC3 antibody

  DNJC3_HUMAN antibodydouble-stranded RNA-activated protein kinase inhibitor antibodyEndoplasmic reticulum DnaJ protein 6 antibodyERdj6 antibodyHP58 antibodyInterferon induced, double stranded RNA activated protein kinase inhibitor antibodyInterferon-induced antibodyP58 antibodyp58 ipk antibodyP58IPK antibodyPRKRI antibodyProtein kinase inhibitor of 58 kDa antibodyProtein kinase inhibitor p58 antibodyProtein kinase interferon inducible double stranded RNA dependent inhibitor antibody

  see all

Anti-DNAJC3 antibody images

• 

  Western blot - DNAJC3 antibody (ab72885)
  All lanes : Anti-DNAJC3 antibody (ab72885) at 1 µg/ml
  
  Lane 1 : TE 671 (Human Rhabdomyosarcoma) Whole Cell Lysate
  Lane 2 : Y79 (Human retinoblastoma cell line) Whole Cell Lysate
  Lane 3 : Hela Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
  Lane 4 : Jurkat Whole Cell Lysate - Staurosporine Treated (24hr, 500nM)
  Lane 5 : Thymus (Rat) Tissue Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 58 kDa
  Observed band size : 54 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunocytochemistry/ Immunofluorescence - DNAJC3 antibody (ab72885)
  ICC/IF image of ab72885 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab72885, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% PFA fixed (10 min) Hek293, HepG2, MCF-7 cells at 1µg/ml, and in 100% Methanol fixed (5 min) HeLa, HepG2, Hek293, MCF-7 cells at 1µg/ml.