Anti-RbAp48 antibody (ab74188)

Overview

• Product nameAnti-RbAp48 antibodySee all RbAp48 primary antibodies ...
• Description
  Rabbit polyclonal to RbAp48
• Tested applicationsWB, IP more details
• Species reactivity
  Reacts with: Mouse, Human
  
• Immunogen

  Synthetic peptide corresponding to a region between residue 1 and 50 of human RbAp48 (CAA52321.1).

• Positive controlHeLa, 293T and NIH3T3 whole cell lysates.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: 0.1% BSA, Tris buffered saline
• Concentration information loading...
• PurityImmunogen affinity purified
• Purification notesab74188 was affinity purified using an epitope specific to RbAp48 immobilized on solid support.
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Cancer
  • Oncoproteins/suppressors
  • Tumor suppressors
  • Rb family

  • Epigenetics and Nuclear Signaling
  • Chromatin Modifying Enzymes
  • Acetylation
  • HDACs
  • Class I

  • Epigenetics and Nuclear Signaling
  • Chromatin Remodeling
  • Polycomb Silencing
  • PRC2

  • Epigenetics and Nuclear Signaling
  • Cell cycle
  • Cell Cycle Inhibitors
  • Rb

  • Epigenetics and Nuclear Signaling
  • Chromatin Remodeling
  • ISWI/NURF/CHRAC

Applications

Target

• FunctionCore histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair; the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression; the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling; the PRC2/EED-EZH2 complex, which promotes repression of homeotic genes during development; and the NURF (nucleosome remodeling factor) complex.
• Sequence similaritiesBelongs to the WD repeat RBAP46/RBAP48/MSI1 family.
  Contains 6 WD repeats.
• Cellular localizationNucleus.

Target information above from: UniProt accession Q09028 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 5928 Human
  • Entrez Gene: 19646 Mouse
  • Omim: 602923 Human
  • SwissProt: Q09028 Human
  • SwissProt: Q60972 Mouse
  • Unigene: 16003 Human
  • Unigene: 12145 Mouse

• Alternative names
  CAF 1 subunit C antibodyCAF I 48 kDa subunit antibodyCAF I p48 antibody

  CAF-1 subunit C antibodyCAF-I 48 kDa subunit antibodyCAF-I p48 antibodyChromatin assembly factor 1 subunit C antibodyChromatin assembly factor I p48 subunit antibodyChromatin assembly factor/CAF 1 p48 subunit antibodyHistone binding protein RBBP4 antibodyHistone-binding protein RBBP4 antibodyMSI1 protein homolog antibodyNucleosome remodeling factor subunit RBAP48 antibodyNucleosome-remodeling factor subunit RBAP48 antibodyNURF 55 antibodyNURF55 antibodyRbAp 48 antibodyRBAP48 antibodyRBBP 4 antibodyRBBP-4 antibodyRBBP4 antibodyRBBP4_HUMAN antibodyRetinoblastoma binding protein 4 antibodyRetinoblastoma binding protein p48 antibodyRetinoblastoma-binding protein 4 antibodyRetinoblastoma-binding protein p48 antibody

  see all

Anti-RbAp48 antibody images

• 

  Western blot - RbAp48 antibody (ab74188)
  All lanes : Anti-RbAp48 antibody (ab74188) at 0.04 µg/ml
  
  Lane 1 : HeLa whole cell lysate at 50 µg
  Lane 2 : HeLa whole cell lysate at 15 µg
  Lane 3 : HeLa whole cell lysate at 5 µg
  Lane 4 : 293T whole cell lysate at 50 µg
  Lane 5 : NIH3T3 whole cell lysate at 50 µg
  
  developed using the ECL technique
  
  Predicted band size : 48 kDa
  Observed band size : 52 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 70 kDa. We are unsure as to the identity of these extra bands.
  
  Exposure time : 30 seconds
• 

  Immunoprecipitation - RbAp48 antibody (ab74188)
  Detection of RbAp48 by Western Blot of Immunprecipitate.
  ab74188, at 0.1µg/ml, staining RbAp48 in HeLa whole cell lysate immunoprecipitated using ab74188 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
  Detection: Chemiluminescence with exposure time of 10 seconds.