Anti-MT-ND1 antibody (ab74257)

Overview

• Product nameAnti-MT-ND1 antibody
• Description
  Rabbit polyclonal to MT-ND1
• SpecificityThis antibody detects endogenous levels of total MT-ND1 protein.
• Tested applicationsWB, ELISA, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Human, Fruit fly (Drosophila melanogaster)
  
• Immunogen

  A synthesized peptide derived from an internal region of human MT-ND1.

• Positive controlHuman placenta tissue and extracts from Jurkat cells.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 50% Glycerol, PBS (without Mg²⁺ and Ca²⁺), 150mM Sodium chloride, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Integration of energy

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Oxidative phosphorylation
  • Complex I

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Integration of energy metabolism

  • Signal Transduction
  • Metabolism
  • Mitochondrial

Applications

Target

• FunctionCore subunit of the mitochondrial membrane respiratory chain NADH dehydrogenase (Complex I) that is believed to belong to the minimal assembly required for catalysis. Complex I functions in the transfer of electrons from NADH to the respiratory chain. The immediate electron acceptor for the enzyme is believed to be ubiquinone.
• Involvement in diseaseDefects in MT-ND1 are a cause of Leber hereditary optic neuropathy (LHON) [MIM:535000]. LHON is a maternally inherited disease resulting in acute or subacute loss of central vision, due to optic nerve dysfunction. Cardiac conduction defects and neurological defects have also been described in some patients. LHON results from primary mitochondrial DNA mutations affecting the respiratory chain complexes.
  Defects in MT-ND1 are a cause of mitochondrial encephalomyopathy with lactic acidosis and stroke-like episodes syndrome (MELAS) [MIM:540000]. MELAS is a genetically heterogenious disorder, characterized by episodic vomiting, seizures, and recurrent cerebral insults resembling strokes and causing hemiparesis, hemianopsia, or cortical blindness.
  Defects in MT-ND1 may be associated with susceptibility to Alzheimer disease mitochondrial (AD-MT) [MIM:502500]. Alzheimer disease is a neurodegenerative disorder characterized by progressive dementia, loss of cognitive abilities, and deposition of fibrillar amyloid proteins as intraneuronal neurofibrillary tangles, extracellular amyloid plaques and vascular amyloid deposits. The major constituent of these plaques is the neurotoxic amyloid-beta-APP 40-42 peptide (s), derived proteolytically from the transmembrane precursor protein APP by sequential secretase processing. The cytotoxic C-terminal fragments (CTFs) and the caspase-cleaved products such as C31 derived from APP, are also implicated in neuronal death.
  Defects in MT-ND1 may be associated with non-insulin-dependent diabetes mellitus (NIDDM).
• Sequence similaritiesBelongs to the complex I subunit 1 family.
• Cellular localizationMitochondrion inner membrane.

Target information above from: UniProt accession P03886 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 192473 Fruit fly (Drosophila melanogaster)
  • Entrez Gene: 4535 Human
  • Entrez Gene: 17716 Mouse
  • SwissProt: P18929 Fruit fly (Drosophila melanogaster)
  • SwissProt: P03886 Human
  • SwissProt: P03888 Mouse

• Alternative names
  Mitochondrially encoded NADH dehydrogenase 1 antibodyMT-ND1 antibodyMTND1 antibody

  NAD1 antibodyNADH dehydrogenase subunit 1 (complex I) antibodyNADH dehydrogenase subunit 1 antibodyNADH-ubiquinone oxidoreductase chain 1 antibodyNADH1 antibodyND1 antibodyNU1M_HUMAN antibody

  see all

Anti-MT-ND1 antibody images

• 

  Western blot - MT-ND1 antibody (ab74257)
  All lanes : Anti-MT-ND1 antibody (ab74257) at 1/500 dilution
  
  Lane 1 : Extracts from Jurkat cells
  Lane 2 : Extracts from Jurkat cells plus 5µg immunizing peptide
  
  Lysates/proteins at 5 µg per lane.
  
  
  Predicted band size : 36 kDa
  Observed band size : 36 kDa
  Additional bands at : 21 kDa,55 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MT-ND1 antibody (ab74257)
  Immunohistochemical analysis of MT-ND1 in paraffin embedded human placenta tissue using ab74257 at a 1/50 dilution.
  Left image: untreated.
  Right image: treated with immunizing peptide.
• 

  Western blot - Anti-MT-ND1 antibody (ab74257)
  All lanes : Anti-MT-ND1 antibody (ab74257) at 1/500 dilution
  
  Lane 1 : Whole larval extract
  Lane 2 : Whole larval extract
  Lane 3 : Whole larval extract
  
  Lysates/proteins at 100 µg per lane.
  
  Secondary
  Donkey polyclonal to Rabbit IgG conjugated to IRDye 800CW at 1/500 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 36 kDa
  
  
  Exposure time : 5 minutes

  Joseph Bateman, Kings College London, United Kingdom

  See Abreview