Anti-beta Actin antibody - Loading Control (ab75186)

Overview

• Product nameAnti-beta Actin antibody - Loading ControlSee all beta Actin primary antibodies ...
• Description
  Rabbit polyclonal to beta Actin - Loading Control
• Tested applicationsWB, IHC-P, ICC/IF more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Synthetic peptide derived from within residues 1 - 100 of Human beta Actin.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab13772.)

• Positive controlThis antibody gave a positive signal in the following whole cell lysates: Jurkat, A431, HEK293, HepG2, MCF-7, NIH3T3 and PC12. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal skin and in HeLa cell line in IF.
• General notesab75186 is raised using the same immunogen as ab8227. Batches sold under ab75186 have passed QC testing in Western blot only. In our hands, this product did not work in ICC/IF. For further information please contact our Customer Services team.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS. pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Signal Transduction
  • Cytoskeleton / ECM
  • Cytoskeleton
  • Microfilaments
  • Actin etc
  • Actin

  • Isotype/Loading Controls
  • Loading Controls
  • Beta Actin

  • Tags & Cell Markers
  • Subcellular Markers
  • Cytoskeleton
  • Actin

Applications

Target

• FunctionActins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
• Involvement in diseaseDefects in ACTB are a cause of dystonia juvenile-onset (DYTJ) [MIM:607371]. DYTJ is a form of dystonia with juvenile onset. Dystonia is defined by the presence of sustained involuntary muscle contraction, often leading to abnormal postures. DYTJ patients manifest progressive, generalized, dopa-unresponsive dystonia, developmental malformations and sensory hearing loss.
• Sequence similaritiesBelongs to the actin family.
• Post-translational
  modificationsISGylated.
• Cellular localizationCytoplasm > cytoskeleton. Localized in cytoplasmic mRNP granules containing untranslated mRNAs.

Target information above from: UniProt accession P60709 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 60 Human
  • Entrez Gene: 11461 Mouse
  • Entrez Gene: 81822 Rat
  • Omim: 102630 Human
  • SwissProt: P60709 Human
  • SwissProt: P60710 Mouse
  • SwissProt: P60711 Rat
  • Unigene: 520640 Human

  • Unigene: 708120 Human
  • Unigene: 727576 Human
  • Unigene: 328431 Mouse
  • Unigene: 391967 Mouse
  • Unigene: 94978 Rat

  see all

• Alternative names
  A X actin like protein antibodyACTB antibodyACTB_HUMAN antibody

  Actin antibodyActin cytoplasmic 1 antibodyActx antibodyb-actin antibodyBeta cytoskeletal actin antibodyBeta-actin antibodycytoplasmic 1 antibodyMelanoma X actin antibodyN-terminally processed antibodyPS1TP5 binding protein 1 antibodyPS1TP5BP1 antibody

  see all

Anti-beta Actin antibody - Loading Control images

• 

  Western blot - beta Actin antibody - Loading Control (ab75186)
  All lanes : Anti-beta Actin antibody - Loading Control (ab75186) at 1 µg/ml
  
  Lane 1 : Jurkat (Human T cell lymphoblast-like cell line) Whole Cell Lysate
  Lane 2 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
  Lane 3 : HEK293 (Human embryonic kidney cell line) Whole Cell Lysate
  Lane 4 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
  Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
  Lane 6 : NIH 3T3 (Mouse embryonic fibroblast cell line) Whole Cell Lysate (ab7179)
  Lane 7 : PC12 (Rat adrenal pheochromocytoma cell line) Whole Cell Lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  
  Performed under reducing conditions.
  
  Predicted band size : 42 kDa
  Observed band size : 42 kDa
  
• 

  Western blot - Anti-beta Actin antibody - Loading Control (ab75186)
  
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 42 kDa
  
  
  Exposure time : 10 seconds
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-beta Actin antibody - Loading Control (ab75186)

  IHC image of beta Actin staining in Human normal skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab75186, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

  For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

• 

  Immunocytochemistry/ Immunofluorescence - Anti-beta Actin antibody - Loading Control (ab75186)
  ICC/IF image of ab75186 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75186, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.