Anti-SOX2 antibody [57CT23.3.4] (ab75485)

Overview

• Product nameAnti-SOX2 antibody [57CT23.3.4]See all SOX2 primary antibodies ...
• Description
  Mouse monoclonal [57CT23.3.4] to SOX2
• Tested applicationsICC/IF, IHC - Wholemount, Flow Cyt, Sandwich ELISA, WB, ELISA, IHC-P more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Recombinant full length SOX2 protein (Human).

• Positive control293 cell lysate transfected with SOX2 gene; human lung carcinoma tissue

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.09% Sodium Azide
  Constituents: PBS
• Concentration information loading...
• PurityProtein G purified
• Clonality Monoclonal
• Clone number57CT23.3.4
• IsotypeIgG1
• Light chain typekappa
• Research Areas
  • Developmental Biology
  • Reproduction
  • Germ cell markers

  • Developmental Biology
  • Embryogenesis
  • Embryonic stem cells
  • Surface molecules

  • Stem Cells
  • Germline Stem Cells
  • Embryonic Germ Cells

  • Stem Cells
  • Embryonic Stem Cells
  • Intracellular

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • HLH / Leucine Zipper
  • Leucine Zipper

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Domain Families
  • HMG Box

  • Neuroscience
  • Neurology process
  • Neurogenesis

  • Neuroscience
  • Cell Type Marker
  • Neural Stem Cell marker

  • Neuroscience
  • Neurology process
  • Neural Signal Transduction

Applications

Target

• FunctionTranscription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
• Involvement in diseaseDefects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
• Sequence similaritiesContains 1 HMG box DNA-binding domain.
• Post-translational
  modificationsSumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
• Cellular localizationNucleus.

Target information above from: UniProt accession P48431 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6657 Human
  • Entrez Gene: 20674 Mouse
  • Entrez Gene: 499593 Rat
  • Omim: 184429 Human
  • SwissProt: P48431 Human
  • SwissProt: P48432 Mouse
  • Unigene: 518438 Human
  • Unigene: 65396 Mouse

  • Unigene: 17369 Rat

  see all

• Alternative names
  ANOP3 antibodycb236 antibodyDelta EF2a antibody

  lcc antibodyMCOPS3 antibodyMGC148683 antibodyMGC2413 antibodyRGD1565646 antibodySex determining region Y box 2 antibodySOX 2 antibodySox2 antibodySOX2_HUMAN antibodySRY (sex determining region Y) box 2 antibodySRY box containing gene 2 antibodySRY related HMG box 2 antibodySRY related HMG box gene 2 antibodySRY-box 2 antibodyTranscription factor SOX 2 antibodyTranscription factor SOX-2 antibodyysb antibody

  see all

Anti-SOX2 antibody [57CT23.3.4] images

• 

  Sandwich ELISA - SOX2 antibody [57CT23.3.4] (ab75485)
  Standard Curve for SOX2 (Analyte: SOX2 protein (Human) (ab79950)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [57CT23.3.4] to SOX2 (ab75485) at 0.2µg/ml and Detector Antibody Rabbit monoclonal [EPR3131] to SOX2 (ab92494) at 0.5µg/ml.
• 

  Western blot - SOX2 antibody [57CT23.3.4] (ab75485)
  Anti-SOX2 antibody [57CT23.3.4] (ab75485) at 1/200 dilution + SOX2 recombinant protein
  
  Predicted band size : 34 kDa
  Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  
• 

  Western blot - SOX2 antibody [57CT23.3.4] (ab75485)
  All lanes : Anti-SOX2 antibody [57CT23.3.4] (ab75485) at 1/200 dilution
  
  Lane 1 : non-transfected 293 cell lysate
  Lane 2 : lysate of 293 cells transiently transfected with SOX2 gene
  
  Lysates/proteins at 12.5 µg per lane.
  
  
  Predicted band size : 34 kDa
  Observed band size : 36 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - SOX2 antibody [57CT23.3.4] (ab75485)
  Human lung carcinoma tissue with ab75485 at 1/50 dilution, using a peroxidase-conjugated secondary antibody, followed by DAB staining.
• 

  Immunocytochemistry/ Immunofluorescence - SOX2 antibody [57CT23.3.4] (ab75485)This image was kindly supplied by Dr David Parkinson by Abreview
  ab75485 staining SOX2 in human schwann cells by Immunocytochemistry/ Immunofluorescence. The cells were fixed in 4% paraformaldehyde for 10 minutes, permeabilised in 0.2% Triton X-100 and then blocked using 10% DCS/ 0.1M lysine/PBS for 30 minutes at 25°C. Samples were then incubated with primary antibody at 1/100 for 16 hours at 4°C. The secondary antibody used was a rabbit anti-mouse IgG conjugated to Alexa Fluor® 568 used at a 1/500 dilution. Cells were counterstained with Hoechst dye to show nuclei.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - SOX2 antibody [57CT23.3.4] (ab75485)Ruma Raha-Chowdhury, University Of Cambridge, United Kingdom

  Immunocytochemistical detection of SOX2 antibody (ab75485) on a mixed rat glial cell culture. Fixative: Paraformaldehyde Permeabilization: 0.3x Triton. Blocking step: 10% serum for 1 hour @ 24°C. Primary antibody used at 1 µg/ml incubated for 24 hours @ 4°C. Secondary antibody: Alexa Fluor® 568 (1/1000). The submitted image shows rat primary glial culture stained with ab75485; DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  See Abreview

• 

  Immunocytochemistry/ Immunofluorescence - SOX2 antibody [57CT23.3.4] (ab75485)
  ICC/IF image of ab75485 stained mouse embryonic stem cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75485, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
  
  
• 

  Flow Cytometry - Anti-SOX2 antibody [57CT23.3.4] (ab75485)
  Overlay histogram showing F9 cells stained with ab75485 (red line). The cells were fixed with 80% methanol (5 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab75485, 2µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 4% paraformaldehyde (10 min) fixed F9 cells used under the same conditions.
  
  Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.
  

Protocols

Protocol Booklet