Anti-Cathepsin D antibody [EPR3056Y] (ab75811)

Overview

• Product nameAnti-Cathepsin D antibody [EPR3056Y]See all Cathepsin D primary antibodies ...
• Description
  Rabbit monoclonal [EPR3056Y] to Cathepsin D
• Tested applicationsWB, IHC-P, ICC more details
• Species reactivity
  Reacts with: Human
  

  Does not react with

  Mouse, Rat
• Immunogen

  A synthetic peptide corresponding to residues near the N-terminus of human Cathepsin D

• Positive controlMCF-7 and SKBR3 cell lysates. Human liver tissue.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR3056Y
• IsotypeIgG
• Research Areas
  • Cancer
  • Invasion/microenvironment
  • ECM
  • Extracellular matrix
  • Other

  • Signal Transduction
  • Cytoskeleton / ECM
  • Extracellular Matrix
  • ECM Enzymes
  • Other Enzymes

  • Neuroscience
  • Cell Adhesion Proteins
  • Membrane Proteins

  • Cell Biology
  • Proteolysis / Ubiquitin
  • Proteolytic enzymes
  • Cysteine protease
  • Cathepsins

Applications

Target

• FunctionAcid protease active in intracellular protein breakdown. Involved in the pathogenesis of several diseases such as breast cancer and possibly Alzheimer disease.
• Tissue specificityExpressed in the aorta extrcellular space (at protein level).
• Involvement in diseaseNeuronal ceroid lipofuscinosis 10 (CLN10) [MIM:610127]: A form of neuronal ceroid lipofuscinosis with onset at birth or early childhood. Neuronal ceroid lipofuscinoses are progressive neurodegenerative, lysosomal storage diseases characterized by intracellular accumulation of autofluorescent liposomal material, and clinically by seizures, dementia, visual loss, and/or cerebral atrophy. Note=The disease is caused by mutations affecting the gene represented in this entry.
• Sequence similaritiesBelongs to the peptidase A1 family.
• Post-translational
  modificationsN- and O-glycosylated.
• Cellular localizationLysosome. Melanosome. Secreted > extracellular space. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. In aortic samples, detected as an extracellular protein loosely bound to the matrix.

Target information above from: UniProt accession P07339 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 1509 Human
  • Omim: 116840 Human
  • SwissProt: P07339 Human
  • Unigene: 654447 Human

• Alternative names
  CatD antibodyCATD_HUMAN antibodyCathepsin D heavy chain antibody

  Cathepsin D light chain antibodyCathepsinD antibodyCD antibodyCeroid lipofuscinosis neuronal 10 antibodyCLN10 antibodyCPSD antibodyCTSD antibodyLysosomal aspartyl peptidase antibodyLysosomal aspartyl protease antibodyMGC2311 antibody

  see all

Anti-Cathepsin D antibody [EPR3056Y] images

• 

  Western blot - Cathepsin D antibody [EPR3056Y] (ab75811)
  All lanes : Anti-Cathepsin D antibody [EPR3056Y] (ab75811) at 1/1000 dilution
  
  Lane 1 : MCF-7 cell lysate
  Lane 2 : SKBR3 cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 46 kDa
  Observed band size : 15,46 kDa (why is the actual band size different from the predicted?)
  
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Cathepsin D antibody [EPR3056Y] (ab75811)
  Immunohistochemical analysis of paraffin-embedded human liver using ab75811 at 1/500 dilution.