Anti-HtrA2 / Omi antibody [EPR22] (ab75982)

Overview

• Product nameAnti-HtrA2 / Omi antibody [EPR22]See all HtrA2 / Omi primary antibodies ...
• Description
  Rabbit monoclonal [EPR22] to HtrA2 / Omi
• Tested applicationsWB, IP, IHC-P, ICC, Flow Cyt more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  A synthetic peptide corresponding to residues on human HtrA2

• Positive controlJurkat cell lysate, 293T cell lysate, HeLa cell lysate and Human colon tissue.
• General notesProduced under U.S. Patent No. 5,675,063.

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
• Storage bufferPBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%
• PurityTissue culture supernatant
• Clonality Monoclonal
• Clone numberEPR22
• IsotypeIgG
• Research Areas
  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Oxidative stress

  • Metabolism
  • Pathways and Processes
  • Mitochondrial Metabolism
  • Mitochondrial markers

  • Metabolism
  • Pathways and Processes
  • Metabolism processes
  • Apoptosis

  • Cancer
  • Invasion/microenvironment
  • Apoptosis
  • Other

  • Neuroscience
  • Neurology process
  • Neurodegenerative disease
  • Parkinson's disease
  • Parkin / PARK

  • Cell Biology
  • Other Antibodies
  • Oxidative Stress

  • Cell Biology
  • Apoptosis
  • Mitochondrial

Applications

Target

• FunctionSerine protease that shows proteolytic activity against a non-specific substrate beta-casein. Promotes or induces cell death either by direct binding to and inhibition of BIRC proteins (also called inhibitor of apoptosis proteins, IAPs), leading to an increase in caspase activity, or by a BIRC inhibition-independent, caspase-independent and serine protease activity-dependent mechanism. Cleaves THAP5 and promotes its degradation during apoptosis. Isoform 2 seems to be proteolytically inactive.
• Tissue specificityIsoform 1 is ubiquitous. Isoform 2 is expressed predominantly in the kidney, colon and thyroid.
• Involvement in diseaseDefects in HTRA2 are the cause of Parkinson disease type 13 (PARK13) [MIM:610297]. A complex neurodegenerative disorder characterized by bradykinesia, resting tremor, muscular rigidity and postural instability, as well as by a clinically significant response to treatment with levodopa. The pathology involves the loss of dopaminergic neurons in the substantia nigra and the presence of Lewy bodies (intraneuronal accumulations of aggregated proteins), in surviving neurons in various areas of the brain.
• Sequence similaritiesBelongs to the peptidase S1B family.
  Contains 1 PDZ (DHR) domain.
• DomainThe mature N-terminus is involved in the interaction with XIAP.
  The PDZ domain mediates interaction with MXI2.
• Post-translational
  modificationsAutoproteolytically activated.
• Cellular localizationMitochondrion intermembrane space. Mitochondrion membrane. Predominantly present in the intermembrane space. Released into the cytosol following apoptotic stimuli, such as UV treatment, and stimulation of mitochondria with caspase-8 truncated BID/tBID.

Target information above from: UniProt accession O43464 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 27429 Human
  • Entrez Gene: 64704 Mouse
  • Entrez Gene: 297376 Rat
  • Omim: 606441 Human
  • SwissProt: O43464 Human
  • SwissProt: Q9JIY5 Mouse
  • Unigene: 469045 Human
  • Unigene: 21880 Mouse

• Alternative names
  High temperature requirement protein a2 antibodyHTRA 2 antibodyHtrA like serine protease antibody

  HtrA serine peptidase 2 antibodyHtrA2 antibodyHTRA2_HUMAN antibodymitochondrial antibodyOmi stress regulated endoprotease antibodyOmi stress-regulated endoprotease antibodyPARK 13 antibodyPARK13 antibodyProtease serine 25 antibodyPRSS 25 antibodyPRSS25 antibodySerine protease 25 antibodySerine protease HTRA2 antibodySerine protease HTRA2 mitochondrial antibodySerine protease htra2 mitochondrial precursor antibodySerine protease omi antibodySerine proteinase OMI antibody

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Anti-HtrA2 / Omi antibody [EPR22] images

• 

  Western blot - HtrA2 / Omi antibody [EPR22] (ab75982)
  All lanes : Anti-HtrA2 / Omi antibody [EPR22] (ab75982) at 1/5000 dilution
  
  Lane 1 : Jurkat cell lysate
  Lane 2 : 293T cell lysate
  Lane 3 : HeLa cell lysate
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  HRP labelled goat anti-rabbit at 1/2000 dilution
  
  Predicted band size : 49 kDa
  Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  Additional bands at : 43 kDa. We are unsure as to the identity of these extra bands.
• 

  Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - HtrA2 / Omi antibody [EPR22] (ab75982)
  Immunohistochemical analysis of paraffin-embedded human colon using ab75982 at 1/100-1/250 dilution.
• 

  - Anti-HtrA2 / Omi antibody [EPR22] (ab75982)

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