Anti-N Cadherin antibody (ab76057)
Overview
- Product nameAnti-N Cadherin antibodySee all N Cadherin primary antibodies ...
- DescriptionRabbit polyclonal to N Cadherin
- Specificityab76057 does not crossreact with E Cadherin.
- Tested applicationsWB, IP, ELISA, ICC/IF, Flow Cyt more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Chimpanzee - Immunogen
Unphosphorylated synthetic peptide: ERPIHAEPQYPVRS, corresponding to amino acids 811-824 of Human N Cadherin (coupled to a carrier protein). This sequence is conserved in rat and mouse N cadherin, and has three amino acid differences from the conserved region in R Cadherin.
- Positive controlWB: Human endothelial cells (untreated or treated with pervanadate (1 mM) for 30 minutes) and mouse brain tissue. This antibody gave a positive result in IHC in the following FFPE tissue: Human normal heart muscle.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
- Storage bufferPreservative: 0.05% Sodium Azide
Constituents: 50% Glycerol, PBS, 1mg/ml BSA -
Concentration information loading... - PurityImmunogen affinity purified
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab76057 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | |
| IP | |
| ELISA | |
| ICC/IF | |
| Flow Cyt |
Flow Cyt: 1/100.
IP: 1/100.
WB: 1/1000. Predicted molecular weight: 100 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionCadherins are calcium dependent cell adhesion proteins. They preferentially interact with themselves in a homophilic manner in connecting cells; cadherins may thus contribute to the sorting of heterogeneous cell types. CDH2 may be involved in neuronal recognition mechanism. In hippocampal neurons, may regulate dendritic spine density.
- Sequence similaritiesContains 5 cadherin domains.
- Cellular localizationCell membrane.
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Database links
- Entrez Gene: 414745 Chicken
- Entrez Gene: 1000 Human
- Entrez Gene: 12558 Mouse
- Entrez Gene: 83501 Rat
- Omim: 114020 Human
- SwissProt: P10288 Chicken
- SwissProt: P19022 Human
- SwissProt: P15116 Mouse
- SwissProt: Q9Z1Y3 Rat
- Unigene: 464829 Human
- Unigene: 606106 Human
- Unigene: 257437 Mouse
- Unigene: 23200 Rat
see all
Target information above from: UniProt accession
P19022
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- CADH2_HUMAN antibodyCadherin 2 antibodyCadherin 2 N cadherin neuronal antibody
- Cadherin 2 type 1 antibodycadherin 2 type 1 N cadherin neuronal antibodyCadherin 2, type 1, N-cadherin (neuronal) antibodyCadherin-2 antibodyCadherin2 antibodyCalcium dependent adhesion protein neuronal antibodyCD325 antibodyCD325 antigen antibodyCDH2 antibodyCDHN antibodyCDw325 antibodyCDw325 antigen antibodyN cadherin 1 antibodyN-cadherin antibodyNCAD antibodyNeural Cadherin antibodyOTTHUMP00000066304 antibodyOTTHUMP00000067378 antibody
see all
Anti-N Cadherin antibody images
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Western blot - N Cadherin antibody (ab76057)All lanes : Anti-N Cadherin antibody (ab76057) at 1/1000 dilution
Lane 1 : Human endothelial cells untreated
Lane 2 : Human endothelial cells treated with pervanadate (1 mM) for 30 min
Predicted band size : 100 kDa
Observed band size : 130 kDa (why is the actual band size different from the predicted?) -
Immunocytochemistry/ Immunofluorescence - N Cadherin antibody (ab76057)ab76057 at 1:100, staining differentiated embryonic stem cells by ICC/IF -
Western blot - N Cadherin antibody (ab76057)All lanes : Anti-N Cadherin antibody (ab76057) at 1/1000 dilution
Lane 1 : MDA-MB-231 cells (Negative Control)
Lane 2 : U2OS cells (Positive Control)
Lysates/proteins at 200000 cells per lane.
Secondary
Goat anti-rabbit HRP at 1/2000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 100 kDa
Observed band size : 100 kDa
Exposure time : 5 minutesThis image is courtesy of an anonymous Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-N Cadherin antibody (ab76057)IHC image of N Cadherin staining in Human normal heart muscle formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab76057, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
References for Anti-N Cadherin antibody (ab76057)
This product has been referenced in:
- Chang CJ et al. p53 regulates epithelial-mesenchymal transition and stem cell properties through modulating miRNAs. Nat Cell Biol 13:317-23 (2011). WB ; Human . Read more (PubMed: 21336307) »
