Anti-XBP1 antibody (ab76443)

Overview

• Product nameAnti-XBP1 antibodySee all XBP1 primary antibodies ...
• Description
  Mouse monoclonal to XBP1
• Tested applicationsELISA, WB more details
• Species reactivity
  Reacts with: Human, Recombinant Fragment
  
• Immunogen

  Recombinant XBP1 fragment (Human) (AAH12841, 123 a.a. ~ 225 a.a) with a tag.

• Positive controlHela NE cell lysate

Properties

• FormLiquid
• Storage instructionsStore at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
• Storage bufferPreservative: None
  Constituents: 1X PBS, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• IsotypeIgG2a
• Light chain typekappa
• Research Areas
  • Epigenetics and Nuclear Signaling
  • Transcription
  • Transcription Factors

  • Epigenetics and Nuclear Signaling
  • Transcription
  • Other factors

Applications

Target

• FunctionTranscription factor essential for hepatocyte growth, the differentiation of plasma cells, the immunoglobulin secretion, and the unfolded protein response (UPR). Acts during endoplasmic reticulum stress (ER) by activating unfolded protein response (UPR) target genes via direct binding to the UPR element (UPRE). Binds DNA preferably to the CRE-like element 5'-GATGACGTG[TG]N(3)[AT]T-3', and also to some TPA response elements (TRE). Binds to the HLA DR-alpha promoter. Binds to the Tax-responsive element (TRE) of HTLV-I.
• Involvement in diseaseGenetic variations in XBP1 could be associated with susceptibility to major affective disorder type 7 (MAFD7) [MIM:612371]. Major affective disorders represent a class of mental disorders characterized by a disturbance in mood as their predominant feature.
• Sequence similaritiesBelongs to the bZIP family.
  Contains 1 bZIP domain.
• Cellular localizationNucleus.

Target information above from: UniProt accession P17861 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 7494 Human
  • Omim: 194355 Human
  • SwissProt: P17861 Human
  • Unigene: 437638 Human

• Alternative names
  Tax responsive element binding protein 5 antibodyTax-responsive element-binding protein 5 antibodyTREB5 antibody

  X box binding protein 1 antibodyX box binding protein 2 antibodyX-box-binding protein 1 antibodyXBP 1 antibodyXBP-1 antibodyXBP1 antibodyXBP1_HUMAN antibodyXBP2 antibody

  see all

Anti-XBP1 antibody images

• 

  Western blot - XBP1 antibody (ab76443)
  Anti-XBP1 antibody (ab76443) at 1/500 dilution + Recombinant protein at 0.2 µg
  
  Secondary
  Goat anti-mouse IgG (H&L)-HRP conjugate at 1/5000 dilution
  
  Predicted band size : 29 kDa
  Observed band size : 37 kDa (why is the actual band size different from the predicted?)
  
• 

  Western blot - XBP1 antibody (ab76443)
  Anti-XBP1 antibody (ab76443) at 1/500 dilution + Hela NE cell lysate at 25 µg
  
  Secondary
  Goat anti-mouse IgG (H&L)-HRP conjugate at 1/2500 dilution
  
  Predicted band size : 29 kDa
  Observed band size : 40 kDa (why is the actual band size different from the predicted?)