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Overview

  • Product nameAnti-REEP5 antibodySee all REEP5 primary antibodies ...
  • Description
    Mouse monoclonal to REEP5
  • Tested applicationsWB, ELISA, ICC/IF more details
  • Species reactivity
    Reacts with: Human, Xenopus laevis
  • Immunogen

    Recombinant fragment: KCGFLLWCMA PSPSNGAELL YKRIIRPFFL KHESQMDSVV KDLKDKAKET ADAITKEAKK ATVNLLGEEK KS, corresponding to amino acids 113-185 of Human REEP5, with tag

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

Applications

Our Abpromise guarantee covers the use of ab76451 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB
ELISA
ICC/IF
  • Application notesELISA: Use at an assay dependent dilution.
    WB: 1/500 - 1/1000. Predicted molecular weight: 21 kDa.
    Note: This antibody has only been tested in WB against the recombinant fragment used as immunogen. We have no data on the detection of endogenous protein.

    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • Target

    • Alternative names
        C5orf18 antibodyD5S346 antibodyDP1 antibody
        Polyposis locus protein 1 antibodyProtein TB2 antibodyReceptor accessory protein 5 antibodyReceptor expression enhancing protein 5 antibodyTB2 antibodyTB2 protein antibodyYOP1 antibody
      see all

    Anti-REEP5 antibody images

    • Western blot - REEP5 antibody [3G11] (ab76451)
      Western blot - REEP5 antibody [3G11] (ab76451)
      Anti-REEP5 antibody (ab76451) at 1/500 dilution + Recombinant protein at 0.2 µg

      Secondary
      Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution

      Predicted band size : 21 kDa
      Observed band size : 34 kDa (why is the actual band size different from the predicted?)

    References for Anti-REEP5 antibody (ab76451)

    ab76451 has not yet been referenced specifically in any publications.

    Publishing research using ab76451 ? Please let us know so that we can cite the reference in this datasheet

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    Immunocytochemistry/ Immunofluorescence abreview for Anti-REEP5 antibody

     Good
    Abreviews
    Application Immunocytochemistry/ Immunofluorescence
    Sample Xenopus laevis Cell (meiotic egg extract)
    Specification meiotic egg extract
    Fixative Methanol
    Permeabilization No
    Blocking step BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 25°C
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    Verified customer

    Submitted Feb 22 2011

    Western blot abreview for Anti-REEP5 antibody

     Good
    Abreviews
    Application Western blot
    Sample Xenopus laevis Cell lysate - whole cell (meiotic egg extract)
    Loading amount 25 µg
    Specification meiotic egg extract
    Gel Running Conditions Reduced Denaturing (15%)
    Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    Verified customer

    Submitted Dec 14 2010

    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"