Anti-ACSL1 antibody (ab76702)

Overview

• Product nameAnti-ACSL1 antibodySee all ACSL1 primary antibodies ...
• Description
  Rabbit polyclonal to ACSL1
• Tested applicationsICC/IF, WB more details
• Species reactivity
  Reacts with: Human
  Predicted to work with: Cow, Pig, Orangutan
• Immunogen

  Synthetic peptide conjugated to KLH derived from within residues 1 - 100 of Human ACSL1.

  Read Abcam's proprietary immunogen policy

  (Peptide available as ab90244.)

• Positive controlThis antibody gave a positive signal in the following human tissue lysates: Liver; Kidney; Heart.

Properties

• FormLiquid
• Storage instructionsStore at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: 0.02% Sodium Azide
  Constituents: 1% BSA, PBS, pH 7.4
• Concentration information loading...
• PurityImmunogen affinity purified
• Clonality Polyclonal
• IsotypeIgG
• Research Areas
  • Metabolism
  • Types of disease
  • Cancer

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Lipid metabolism

  • Metabolism
  • Pathways and Processes
  • Redox metabolism
  • Fatty acid oxidation

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Energy transfer pathways
  • Integration of energy

  • Metabolism
  • Pathways and Processes
  • Metabolic signaling pathways
  • Lipid and lipoprotein metabolism
  • Fatty acids

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Integration of energy metabolism

  • Cancer
  • Cancer Metabolism
  • Metabolic signaling pathway
  • Metabolism of lipids and lipoproteins

  • Signal Transduction
  • Metabolism
  • Lipid metabolism

  • Cardiovascular
  • Lipids / Lipoproteins
  • Fatty Acids
  • Metabolism

Applications

Target

• FunctionActivation of long-chain fatty acids for both synthesis of cellular lipids, and degradation via beta-oxidation. Preferentially uses palmitoleate, oleate and linoleate.
• Tissue specificityHighly expressed in liver, heart, skeletal muscle, kidney and erythroid cells, and to a lesser extent in brain, lung, placenta and pancreas.
• Sequence similaritiesBelongs to the ATP-dependent AMP-binding enzyme family.
• Developmental stageExpressed during the early stages of erythroid development while expression is very low in reticulocytes and young erythrocytes.
• Cellular localizationMitochondrion outer membrane. Peroxisome membrane. Microsome membrane. Endoplasmic reticulum membrane.

Target information above from: UniProt accession P33121 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 2180 Human
  • Omim: 152425 Human
  • SwissProt: P33121 Human
  • Unigene: 406678 Human

• Alternative names
  ACS 1 antibodyACS1 antibodyACSL 1 antibody

  ACSL1 antibodyACSL1_HUMAN antibodyAcyl CoA synthetase 1 antibodyAcyl CoA synthetase long chain family member 1 antibodyAcyl-CoA synthetase 1 antibodyFACL 1 antibodyFACL 2 antibodyFACL1 antibodyFACL2 antibodyFatty acid Coenzyme A ligase long chain 1 antibodyFatty acid Coenzyme A ligase long chain 2 antibodyLACS 1 antibodyLACS 2 antibodyLACS antibodyLACS1 antibodyLACS2 antibodyLignoceroyl CoA synthase antibodyLong chain acyl CoA synthetase 1 antibodyLong chain acyl CoA synthetase 2 antibodyLong chain fatty acid CoA ligase 1 antibodyLong chain fatty acid CoA ligase 2 antibodyLong chain fatty acid coenzyme A ligase 1 antibodyLong-chain acyl-CoA synthetase 1 antibodyLong-chain acyl-CoA synthetase 2 antibodyLong-chain fatty acid-CoA ligase 2 antibodyLong-chain-fatty-acid--CoA ligase 1 antibodyPalmitoyl CoA ligase 1 antibodyPalmitoyl CoA ligase 2 antibodyPalmitoyl-CoA ligase 1 antibodyPalmitoyl-CoA ligase 2 antibody

  see all

Anti-ACSL1 antibody images

• 

  Western blot - ACSL1 antibody (ab76702)
  All lanes : Anti-ACSL1 antibody (ab76702) at 1 µg/ml
  
  Lane 1 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)
  Lane 2 : Kidney (Human) Tissue Lysate - adult normal tissue (ab30203)
  Lane 3 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)
  
  Lysates/proteins at 10 µg per lane.
  
  Secondary
  Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 78 kDa
  Observed band size : 78 kDa
  Additional bands at : 75 kDa (possible isoform).
  
  Exposure time : 3 minutes
• 

  Immunocytochemistry/ Immunofluorescence - Anti-ACSL1 antibody (ab76702)
  ICC/IF image of ab76702 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab76702, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899 Dylight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.