Anti-Synaptotagmin 1 antibody (ab77314)

Overview

• Product nameAnti-Synaptotagmin 1 antibodySee all Synaptotagmin 1 primary antibodies ...
• Description
  Mouse monoclonal to Synaptotagmin 1
• Tested applicationsFlow Cyt, WB, ELISA more details
• Species reactivity
  Reacts with: Mouse, Rat, Human
  
• Immunogen

  Recombinant full length human Synaptotagmin 1 protein (AAH58917) with a 26kDa tag

• Positive controlJurkat cell lysate

Properties

• FormLiquid
• Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
• Storage bufferPreservative: None
  Constituents: 1X PBS, pH 7.2
• Concentration information loading...
• PurityProtein A purified
• Clonality Monoclonal
• IsotypeIgG2a
• Light chain typekappa
• Research Areas
  • Signal Transduction
  • Protein Trafficking
  • Vesicle Transport
  • Regulation

  • Neuroscience
  • Neurotransmission
  • Secretory Vesicles
  • Other

  • Neuroscience
  • Neurotransmission
  • Secretory Vesicles
  • Regulation

  • Neuroscience
  • Cell Type Marker
  • Neuron marker
  • Synapse marker

Applications

Target

• FunctionMay have a regulatory role in the membrane interactions during trafficking of synaptic vesicles at the active zone of the synapse. It binds acidic phospholipids with a specificity that requires the presence of both an acidic head group and a diacyl backbone. A Ca(2+)-dependent interaction between synaptotagmin and putative receptors for activated protein kinase C has also been reported. It can bind to at least three additional proteins in a Ca(2+)-independent manner; these are neurexins, syntaxin and AP2.
• Sequence similaritiesBelongs to the synaptotagmin family.
  Contains 2 C2 domains.
• DomainThe first C2 domain mediates Ca(2+)-dependent phospholipid binding.
  The second C2 domain mediates interaction with SV2A and STN2.
• Cellular localizationCytoplasmic vesicle > secretory vesicle > synaptic vesicle membrane. Cytoplasmic vesicle > secretory vesicle > chromaffin granule membrane. Cytoplasm. Synaptic vesicles and chromaffin granules.

Target information above from: UniProt accession P21579 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
• Database links
  • Entrez Gene: 6857 Human
  • Entrez Gene: 20979 Mouse
  • Entrez Gene: 25716 Rat
  • Omim: 185605 Human
  • SwissProt: P21579 Human
  • SwissProt: P46096 Mouse
  • SwissProt: P21707 Rat
  • Unigene: 310545 Human

  • Unigene: 289702 Mouse
  • Unigene: 216272 Rat

  see all

• Alternative names
  DKFZp781D2042 antibodyP65 antibodySVP65 antibody

  Synaptotagmin I antibodySynaptotagmin-1 antibodySYT antibodySYT1 antibodySYT1_HUMAN antibodySytI antibody

  see all

Anti-Synaptotagmin 1 antibody images

• 

  Western blot - Synaptotagmin 1 antibody (ab77314)
  Anti-Synaptotagmin 1 antibody (ab77314) at 5 µg/ml + immunogen at 0.2 µg
  
  Secondary
  Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/5000 dilution
  developed using the ECL technique
  
  Predicted band size : 48 kDa
  Observed band size : 65 kDa (why is the actual band size different from the predicted?)
  
• 

  Western blot - Synaptotagmin 1 antibody (ab77314)
  Anti-Synaptotagmin 1 antibody (ab77314) at 5 µg/ml + Jurkat cell lysate at 25 µg
  
  Secondary
  Goat Anti-Mouse IgG (H&L)-HRP Conjugate at 1/2500 dilution
  developed using the ECL technique
  
  Predicted band size : 48 kDa
  Observed band size : 38 kDa (why is the actual band size different from the predicted?)
  
• 

  ELISA - Synaptotagmin 1 antibody (ab77314)
  ab77314 was used as a capture antibody at 3ng/ml followed by incubation with the immunogen (recombinant protein) at the indicated concentration.
• 

  Flow Cytometry-Anti-Synaptotagmin 1 antibody(ab77314)
  Overlay histogram showing SH-SY5Y cells stained with ab77314 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab77314, 0.5µg/1x10⁶ cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 2µg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.