Overview
- Product nameAnti-Monoacylglycerol Lipase antibodySee all Monoacylglycerol Lipase primary antibodies ...
- DescriptionGoat polyclonal to Monoacylglycerol Lipase
- Specificityabb77398 is expected to recognize both reported isoforms (NP_009214.1 and NP_001003794.1).
- Tested applicationsELISA, IHC-P, WB more details
- Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Dog - Immunogen
Synthetic peptide: C-QDLPHLVNADGQY, corresponding to internal sequence amino acids 17-29 of Human Monoacylglycerol Lipase (NP_009214.1; NP_001003794.1).
- Positive controlHuman Adipose and Brain (Frontal Cortex) lysates.
Properties
- FormLiquid
- Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 0.5% BSA, Tris buffered saline, pH 7.3 -
Concentration information loading... - PurityImmunogen affinity purified
- Purification notesab77398 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab77398 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| ELISA | |
| IHC-P | |
| WB |
WB: Use at a concentration of 0.5 - 1.5 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionConverts monoacylglycerides to free fatty acids and glycerol. Hydrolyzes the endocannabinoid 2-arachidonoylglycerol, and thereby contributes to the regulation of endocannabinoid signaling, nociperception and perception of pain (By similarity). Regulates the levels of fatty acids that serve as signaling molecules and promote cancer cell migration, invasion and tumor growth.
- Tissue specificityDetected in adipose tissue, lung, liver, kidney, brain and heart.
- PathwayGlycerolipid metabolism; triacylglycerol degradation.
- Sequence similaritiesBelongs to the AB hydrolase superfamily. Monoacylglycerol lipase family.
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Database links
- Entrez Gene: 11343 Human
- Entrez Gene: 23945 Mouse
- Entrez Gene: 29254 Rat
- Omim: 609699 Human
- SwissProt: Q99685 Human
- SwissProt: O35678 Mouse
- SwissProt: Q8R431 Rat
- Unigene: 277035 Human
- Unigene: 272197 Mouse
- Unigene: 40396 Rat
see all
Target information above from: UniProt accession
Q99685
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- EC 3.1.1.23 antibodyHU K5 antibodyHU-K5 antibody
- HUK5 antibodyLysophospholipase homolog antibodyLysophospholipase like antibodyLysophospholipase-like antibodyMAGL antibodyMGL antibodyMGLL antibodyMGLL_HUMAN antibodyMonoacylglycerol lipase antibodyMonoglyceride lipase antibody
see all
Anti-Monoacylglycerol Lipase antibody images
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Western blot - Monoacylglycerol Lipase antibody (ab77398)Anti-Monoacylglycerol Lipase antibody (ab77398) at 0.5 µg/ml + Human Adipose lysate (in RIPA buffer) at 35 µg
Predicted band size : 33 kDa
Observed band size : 33 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Monoacylglycerol Lipase antibody (ab77398)Carl Hobbs, King`s College London, United KingdomImmunohistochemical staining of Monoacylglycerol Lipase using ab77398 on Formaldehyde-fixed paraffin-embedded mouse adipose tissue sections (from around the kidney). Antigen retrieval step: heat mediated in citric acid HIER. Blocking step: 1% BSA for 10 mins @ rt°C. ab77398 incubated at 1/500 for 2 hours. Secondary Antibody: biotin labelled anti goat IgG used at 1/200. The submitted image shows white fat deposits around the kidney. Dilution factors vary between 1/300-500. There is a pale positivity in the renal tubular cytoplasm which appears to be specific (at lower dilution factor this is more obvious but, in this block the fat was too strongly immunostained). Section was endogenous biotin-blocked prior to incubation with primary Ab.
References for Anti-Monoacylglycerol Lipase antibody (ab77398)
ab77398 has not yet been referenced specifically in any publications.
