Overview
- Product nameAnti-Piccolo antibodySee all Piccolo primary antibodies ...
- DescriptionRabbit polyclonal to Piccolo
- Tested applicationsWB, IHC-P, IHC-Fr, ICC/IF more details
- Species reactivityReacts with: Mouse, Rat, Human
- Immunogen
Synthetic peptide from an internal sequence of Human Piccolo, conjugated to an immunogenic carrier protein
Properties
- FormLiquid
- Storage instructionsStore at +4°C short term (1-2 weeks). Add glycerol to a final volume of 50%, aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
- Storage bufferPreservative: None
Constituents: Whole serum - PurityWhole antiserum
- Clonality Polyclonal
- IsotypeIgG
- Research Areas
Applications
Our Abpromise guarantee covers the use of ab77496 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Notes |
|---|---|
| WB | |
| IHC-P | |
| IHC-Fr | |
| ICC/IF |
IHC-P: 1/300 - 1/2000.
IHC-Fr: 1/300 - 1/2000. Antigen retrieval is recommended.
WB: 1/300 - 1/2000. Predicted molecular weight: 567 kDa.
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
- FunctionMay act as a scaffolding protein involved in the organization of synaptic active zones and in synaptic vesicle trafficking.
- Sequence similaritiesContains 2 C2 domains.
Contains 1 PDZ (DHR) domain. - DomainC2 domain 1 is involved in binding calcium and phospholipids. Calcium binds with low affinity but with high specificity and induces a large conformational change.
- Cellular localizationCell junction > synapse. Concentrated at the presynaptic side of synaptic junctions.
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Database links
- Entrez Gene: 27445 Human
- Entrez Gene: 26875 Mouse
- Entrez Gene: 56768 Rat
- Omim: 604918 Human
- SwissProt: Q9Y6V0 Human
- SwissProt: Q8CF91 Mouse
- SwissProt: Q8CF92 Mouse
- SwissProt: Q9QYX7 Mouse
- SwissProt: Q9JKS6 Rat
- Unigene: 12376 Human
- Unigene: 146275 Mouse
- Unigene: 53925 Rat
see all
Target information above from: UniProt accession
Q9Y6V0
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010)
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Alternative names
- ACZ antibodyAczonin antibodyBrain-derived HLMN protein antibody
- DKFZp779G1236 antibodyKIAA0559 antibodyMultidomain presynaptic cytomatrix protein antibodyPCLO antibodyPCLO_HUMAN antibodyPiccolo (presynaptic cytomatrix protein) antibodyPiccolo, mouse, homolog of antibodyPico antibodyPresynaptic cytomatrix protein antibodyProtein piccolo antibody
see all
Anti-Piccolo antibody images
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Immunocytochemistry/ Immunofluorescence - Piccolo antibody (ab77496)Immunofluoresence on 1-day-old mouse cortical cell culture using ab77496 at 1/2000 dilution. DAPI counter stain appears in blue.
References for Anti-Piccolo antibody (ab77496)
ab77496 has not yet been referenced specifically in any publications.
