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Anti-CXCR4 antibody (ab77909)

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Overview

  • Product nameAnti-CXCR4 antibodySee all CXCR4 primary antibodies ...
  • Description
    Rabbit polyclonal to CXCR4
  • SpecificityThis antibody detects endogenous levels of total CXCR4 protein.
  • Tested applicationsWB, ELISA, ICC/IF more details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    A synthesized peptide derived from the C terminal of human CXCR4.

  • Positive controlA549 cells and extracts from K562 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab77909 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Notes
WB WB: 1/500 - 1/1000. Detects a band of approximately 35 kDa (predicted molecular weight: 40 kDa).
ELISA ELISA: 1/40000.
ICC/IF ICC/IF: 1/500 - 1/1000.

Target

  • FunctionReceptor for the C-X-C chemokine CXCL12/SDF-1 that transduces a signal by increasing intracellular calcium ions levels and enhancing MAPK1/MAPK3 activation. Acts as a receptor for extracellular ubiquitin; leading to enhance intracellular calcium ions and reduce cellular cAMP levels. Involved in haematopoiesis and in cardiac ventricular septum formation. Plays also an essential role in vascularization of the gastrointestinal tract, probably by regulating vascular branching and/or remodeling processes in endothelial cells. Could be involved in cerebellar development. In the CNS, could mediate hippocampal-neuron survival. Acts as a coreceptor (CD4 being the primary receptor) for HIV-1 X4 isolates and as a primary receptor for some HIV-2 isolates. Promotes Env-mediated fusion of the virus.
  • Tissue specificityExpressed in numerous tissues, such as peripheral blood leukocytes, spleen, thymus, spinal cord, heart, placenta, lung, liver, skeletal muscle, kidney, pancreas, cerebellum, cerebral cortex and medulla (in microglia as well as in astrocytes), brain microvascular, coronary artery and umbilical cord endothelial cells. Isoform 1 is predominant in all tissues tested.
  • Involvement in diseaseDefects in CXCR4 are a cause of WHIM syndrome (WHIM) [MIM:193670]; also known as warts, hypogammaglobulinemia, infections and myelokathexis. WHIM syndrome is an immunodeficiency disease characterized by neutropenia, hypogammaglobulinemia and extensive human papillomavirus (HPV) infection. Despite the peripheral neutropenia, bone marrow aspirates from affected individuals contain abundant mature myeloid cells, a condition termed myelokathexis.
  • Sequence similaritiesBelongs to the G-protein coupled receptor 1 family.
  • DomainThe amino-terminus is critical for ligand binding. Residues in all four extracellular regions contribute to HIV-1 coreceptor activity.
  • Post-translational
    modifications    Phosphorylated on agonist stimulation. Rapidly phosphorylated on serine and threonine residues in the C-terminal. Phosphorylation at Ser-324 and Ser-325 leads to recruitment of ITCH, ubiquitination and protein degradation.
    Ubiquitinated by ITCH at the cell membrane on agonist stimulation. The ubiquitin-dependent mechanism, endosomal sorting complex required for transport (ESCRT), then targets CXCR4 for lysosomal degradation. This process is dependent also on prior Ser-/Thr-phosphorylation in the C-terminal of CXCR4. Also binding of ARRB1 to STAM negatively regulates CXCR4 sorting to lysosomes though modulating ubiquitination of SFR5S.
    Sulfation on Tyr-21 is required for efficient binding of CXCL12/SDF-1alpha and promotes its dimerization.
    O- and N-glycosylated. Asn-11 is the principal site of N-glycosylation. There appears to be very little or no glycosylation on Asn-176. N-glycosylation masks coreceptor function in both X4 and R5 laboratory-adapted and primary HIV-1 strains through inhibiting interaction with their Env glycoproteins. The O-glycosylation chondroitin sulfate attachment does not affect interaction with CXCL12/SDF-1alpha nor its coreceptor activity.
  • Cellular localizationCell membrane. In unstimulated cells, diffuse pattern on plasma membrane. On agonist stimulation, colocalizes with ITCH at the plasma membrane where it becomes ubiquitinated.

    • Target information above from: UniProt accession P61073 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links
    see all
  • Alternative names
      C-X-C chemokine receptor type 4 antibodyCD184 antibodyCD184 antigen antibody
      Chemokine (C X C motif) receptor 4 antibodyChemokine CXC Motif Receptor 4 antibodyCXC-R4 antibodyCXCR-4 antibodyCXCR4 antibodyCXCR4_HUMAN antibodyD2S201E antibodyFB22 antibodyFB22 antibodyFusin antibodyHM89 antibodyHSY3RR antibodyLAP 3 antibodyLAP3 antibodyLCR1 antibodyLESTR antibodyLeukocyte derived seven transmembrane domain receptor antibodyLeukocyte-derived seven transmembrane domain receptor antibodyLipopolysaccharide associated protein 3 antibodyNeuropeptide Y receptor Y3 antibodyNPY3R antibodyNPYR antibodyNPYRL antibodyNPYY3 antibodyNPYY3R antibodyProbable G protein coupled receptor lcr1 homolog antibodySDF 1 receptor antibodySDF-1 receptor antibodySEVEN-TRANSMEMBRANE-SEGMENT RECEPTOR antibodyStromal cell derived factor 1 receptor antibodyStromal cell-derived factor 1 receptor antibodyWHIM antibody
    see all

Anti-CXCR4 antibody images

  • Western blot - CXCR4 antibody (ab77909)
    Western blot - CXCR4 antibody (ab77909)
    All lanes : Anti-CXCR4 antibody (ab77909) at 1/500 dilution

    Lane 1 : Extracts from K562 cells
    Lane 2 : Extracts from K562 cell with immunizing peptide at 10 µg

    Lysates/proteins at 30 µg per lane.


    Predicted band size : 40 kDa
    Observed band size : 40 kDa
  • Immunocytochemistry/ Immunofluorescence - CXCR4 antibody (ab77909)
    Immunocytochemistry/ Immunofluorescence - CXCR4 antibody (ab77909)
    Immunofluorescence analysis of A549 cells using ab77909 at a 1/500 dilution.
    Left image un-treated.
    Right image treated with immunizing peptide.

References for Anti-CXCR4 antibody (ab77909)

ab77909 has not yet been referenced specifically in any publications.

Publishing research using ab77909 ? Please let us know so that we can cite the reference in this datasheet

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Western blot abreview for Anti-CXCR4 antibody

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Abreviews
Application Western blot
Sample Human Cell lysate - whole cell (HeLa, Jurkat)
Loading amount 100 µg
Specification HeLa, Jurkat
Gel Running Conditions Reduced Denaturing (10%)
Blocking step Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 18°C
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Submitted Feb 19 2010

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"