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Overview

  • Product nameAlpha 2 Antiplasmin protein (Active)See all Alpha 2 Antiplasmin proteins and peptides ...
  • Protein descriptionFull length native Alpha 2 Antiplasmin (mature form) purified from human plasma, 70kDa. Swiss ID = P08697.
  • Expression hostNative

    • Properties

  • Purity> 95 % by SDS-PAGE
  • Biological activitySpecific activity: 13.05 IU/mg ab77936 efficiently inhibits the plasminogen-activator-induced lysis of fibrin clots.
  • FormLyophilised:Reconstitution is batch dependent. We deliver 100ug lyophilised product. Please add the correct volume of distilled water to achieve the lot specific concentration indicated on the vial.
  • Storage instructionsStore at -20°C. Stable for 12 months at -20°CThis product is an active protein and may elicit a biological response in vivo, handle with caution.
  • Storage bufferPreservative: None
    Constituents: 200mM Sodium chloride, 20mM Bis-Tris, pH 6.4
  • Concentration information loading...
  • Additional notesAll human source materials have tested negative for HIV1, HIV2, HBc, HCV antibodies and HBsAg by FDA approved tests. No test guarantees a product to be non-infectious. Therefore, all material derived from human fluids or tissues should be considered as potentially infectious.
  • Research Areas

    • Applications

    Our Abpromise guarantee covers the use of ab77936 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Application Notes
    SDS-PAGE SDS-PAGE: Use at an assay dependent dilution.
    WB WB: Use at an assay dependent concentration. Ab77936 can be used as a WB positive control in conjunction with ab62770.

    Protein info

    • Alternative names
        A2APA2AP_HUMANAAP
        Alpha 2 antiplasminAlpha 2 antiplasmin pigment epithelium derived factorAlpha 2 APALPHA 2 PIAlpha 2 plasmin inhibitorAlpha 2 plasmin inhibitor deficiencyAlpha-2-antiplasminAlpha-2-APAlpha-2-PIAlpha-2-plasmin inhibitorAntiplasmin deficiencyAPIPlasmin inhibitor deficiencyPLISerine (or cysteine) peptidase inhibitor, clade F, member 2Serine (Or cysteine) peptidase inhibitor, clade F, member 2, isoform CRA_cSerine (or cysteine) proteinase inhibitor, clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2Serine (Or cysteine) proteinase inhibitor, clade F, member 2Serine or cysteine peptidase inhibitor clade F member 2Serpin F2Serpin peptidase inhibitor clade FSerpin peptidase inhibitor, clade F (alpha 2 antiplasmin pigment epithelium derived factor) member 2SERPINF2
      see all
  • FunctionSerine protease inhibitor. The major targets of this inhibitor are plasmin and trypsin, but it also inactivates matriptase-3/TMPRSS7 and chymotrypsin.
  • Tissue specificityExpressed by the liver and secreted in plasma.
  • Involvement in diseaseDefects in SERPINF2 are the cause of alpha-2-plasmin inhibitor deficiency (APLID) [MIM:262850]. APLID is an autosomal recessive disorder resulting in severe hemorrhagic diathesis.
  • Sequence similaritiesBelongs to the serpin family.
  • Cellular localizationSecreted.

    • Target information above from: UniProt accession P08697 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt

    Alpha 2 Antiplasmin protein (Active) images

    • Western blot
      Western blot
      Anti-Alpha 2 Antiplasmin antibody (ab62770) at 1 µg/ml + Alpha 2 Antiplasmin protein (Active) (ab77936) at 0.01 µg

      Secondary
      Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP), pre-adsorbed (ab97080) at 1/5000 dilution
      developed using the ECL technique

      Performed under reducing conditions.

      Exposure time : 10 seconds

    References for Alpha 2 Antiplasmin protein (Active) (ab77936)

    This product has been referenced in:
    • Wang L  et al. Degradation of Internalized avß5 Integrin Is Controlled by uPAR Bound uPA: Effect on ß1 Integrin Activity and a-SMA Stress Fiber Assembly. PLoS One 7:e33915 (2012). Read more (PubMed: 22470492) »
    • Mollmark J  et al. Antiangiogenic Activity of rPAI-123 Promotes Vasa Vasorum Regression in Hypercholesterolemic Mice Through a Plasmin-Dependent Mechanism. Circ Res 108:1419-28 (2011). Read more (PubMed: 21546607) »

    See all 2 Publications for this product

    Publishing research using ab77936 ? Please let us know so that we can cite the reference in this datasheet

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